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4EBP1 Antibody [Unconjugated]

 
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse 4EBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3227) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for 4EBP1 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse 4EBP1 Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for 4EBP1 at approximately 26 kDa (as indicated) using 5 µg/mL of Goat ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3227-SP
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4EBP1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human 4EBP1
Ser2-Ile118
Accession # Q13541
Specificity
Detects human and mouse 4EBP1.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EIF4EBP1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Knockout Validated
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 4EBP1 Antibody [Unconjugated]

  • 4EBP1
  • 4E-BP1
  • eIF4E-binding protein 1
  • EIF4EBP1
  • eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
  • eukaryotic translation initiation factor 4E-binding protein 1
  • PHAS-I
  • PHAS-IMGC4316
  • Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

The eukaryotic initiation factor 4E (eIF-4E) binding protein 1 (4EBP1) binds to eIF-4E to prevent translation initiation. Upon phosphorylation by the target of rapamycin (TOR) kinase, 4EBP1 releases eIF-4E and translation initiation ensues. 4EBP1 is also known as PHAS-1 (phosphorylated heat and acid stable protein regulated by insulin 1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EIF4EBP1
Uniprot