4EBP1 Antibodies
We offer 4EBP1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each 4EBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. Use the list below to choose the 4EBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our 4EBP1 polyclonal antibodies and browse our 4EBP1 monoclonal antibody catalog.
Alternate Names for 4EBP1 Antibodies
anti-4EBP1 antibody, anti-EIF4EBP1 antibody, anti-eIF4E-binding protein 1 antibody, anti-eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1 antibody, anti-eukaryotic translation initiation factor 4E-binding protein 1 antibody, anti-PHAS-IMGC4316 antibody, anti-Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
16 Results for "4ebp1" in Primary Antibodies
![Knockout Validated: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human 4EBP1 Polyclonal Antibody (Catalog # NB200-157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for 4EBP1 at approximately 17-20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Knockout-Validated-NB200-157-img0011.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human 4EBP1 Polyclonal Antibody (Catalog # NB200-157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for 4EBP1 at approximately 17-20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NB200-157-img0009.jpg)
![Immunohistochemistry: 4EBP1 Antibody [NB200-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20,000 ( 0.05ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-NB200-157-img0010.jpg)
![Western Blot: Rabbit Polyclonal 4EBP1 Antibody [NB200-157] - Whole cell lysates from MDA-MB-231, SUM159 and MCF-7 cells were loaded with 50 ug/lane. 10% SDS-PAGE. 4EBP1 Antibody (NB200-157) was used for primary antibody: 1:2000, 4C, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb200-157_rabbit-polyclonal-4ebp1-antibody-western-blot-311202415623..png)
![Western Blot: 4EBP1 Antibody [NBP1-89368] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP1-89368-img0006.jpg "Western Blot: 4EBP1 Antibody [NBP1-89368] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP1-89368] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89368-img0009.jpg)
![Western Blot: 4EBP1 Antibody [NBP1-89368] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-5](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP1-89368-img0004.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP1-89368] - Staining of human salivary gland shows strong cytoplasmic positivity in cells in serous acini.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89368-img0007.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP1-89368] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89368-img0008.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP1-89368] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89368-img0010.jpg)
![Knockout Validated: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP2-16307-img0004.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NBP2-16307] - 4EBP1 antibody detects 4EBP1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 antibody diluted at a dilution of 1:2000.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP2-16307-img0006.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP2-16307] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/eIF4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16307-img0003.jpg)
![Immunoprecipitation: 4EBP1 Antibody [NBP2-16307] - Immunoprecipitation of 4EBP1 protein from HCT116 whole cell extracts using 5 ug of 4EBP1 antibody or 4E-BP1 antibody. Western blot analysis was performed using 4EBP1 antibody.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunoprecipitation-NBP2-16307-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/4EBP1-Antibody-1E2E7-Immunocytochemistry-Immunofluorescence-NBP2-61900-img0003.jpg "Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Immunohistochemistry: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of paraffin-embedded bladder cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/4EBP1-Antibody-1E2E7-Immunohistochemistry-NBP2-61900-img0004.jpg)
![Flow Cytometry: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/4EBP1-Antibody-1E2E7-Flow-Cytometry-NBP2-61900-img0002.jpg)
![Immunohistochemistry: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of paraffin-embedded esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/4EBP1-Antibody-1E2E7-Immunohistochemistry-NBP2-61900-img0005.jpg)
![ELISA: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/4EBP1-Antibody-1E2E7-ELISA-NBP2-61900-img0001.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] - Wild-type (WT) and 4EBP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13169) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13169-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] - Wild-type (WT) and 4EBP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13169) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13169) diluted at 1:1000.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13169-img0001.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13169) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13169-img0002.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13169) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13169-img0003.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] -Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp3-13169_rabbit-4ebp1-p-thr37-p-thr46-pab-western-blot-273202412343..jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Wild-type (WT) and 4EBP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Wild-type (WT) and 4EBP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:1000.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0001.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0002.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0003.jpg)
![Western Blot: 4EBP1 Antibody (11G12C11) [NBP1-47366] - Analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein (1).](http://images.novusbio.com/fullsize/4EBP1-Antibody-11G12C11-Western-Blot-NBP1-47366-img0005.jpg "Western Blot: 4EBP1 Antibody (11G12C11) [NBP1-47366] - Analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein (1).")
![Immunohistochemistry-Paraffin: 4EBP1 Antibody (11G12C11) [NBP1-47366] - Analysis of human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue, showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/4EBP1-Antibody-11G12C11-Immunohistochemistry-Paraffin-NBP1-47366-img0004.jpg)
![Immunohistochemistry: 4EBP1 Antibody (2D1G11) [NBP2-61901] - Analysis of paraffin-embedded stomach cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/4EBP1-Antibody-2D1G11-Immunohistochemistry-NBP2-61901-img0003.jpg "Immunohistochemistry: 4EBP1 Antibody (2D1G11) [NBP2-61901] - Analysis of paraffin-embedded stomach cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.")
![Flow Cytometry: 4EBP1 Antibody (2D1G11) [NBP2-61901] - Analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/4EBP1-Antibody-2D1G11-Flow-Cytometry-NBP2-61901-img0002.jpg)
![Immunohistochemistry: 4EBP1 Antibody (2D1G11) [NBP2-61901] - Analysis of paraffin-embedded rectum cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/4EBP1-Antibody-2D1G11-Immunohistochemistry-NBP2-61901-img0004.jpg)
![ELISA: 4EBP1 Antibody (2D1G11) [NBP2-61901] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/4EBP1-Antibody-2D1G11-ELISA-NBP2-61901-img0001.jpg)
![Western Blot: 4EBP1 Antibody [NBP1-76621] - eIF [NBP1-76621] - Analysis of eIF4EBP1 in human brain cell lysate with eIF4EBP1 antibody using NBP1-76621 at (A) 2.5, (B) 5 and (C) 10 ug/ml.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP1-76621-img0004.jpg "Western Blot: 4EBP1 Antibody [NBP1-76621] - eIF [NBP1-76621] - Analysis of eIF4EBP1 in human brain cell lysate with eIF4EBP1 antibody using NBP1-76621 at (A) 2.5, (B) 5 and (C) 10 ug/ml.")
![Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody [NBP1-76621] - Staining of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76621-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody [NBP1-76621] - 3T3 cells with eIF4EBP1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76621-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human 4EBP1 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/4EBP1-Antibody-007-Immunocytochemistry-Immunofluorescence-NBP2-89207-img0001.jpg "Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human 4EBP1 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.")
![Western Blot: 4EBP1 Antibody [NBP3-02995] - Analysis of extracts of various cell lines, using eIF4EBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 300s.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP3-02995-img0004.jpg "Western Blot: 4EBP1 Antibody [NBP3-02995] - Analysis of extracts of various cell lines, using eIF4EBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 300s.")