Choose from nearly 1,000 secondary antibodies conjugated to more than 40 different enzymatic, fluorescent, or biotin labels. Use the filters on the left to sort our secondary antibodies by species, application, host, or conjugate.
To learn more about secondary antibodies, download and read our Secondary Antibody Handbook for technical information and tips to guide you when choosing a secondary antibody for your experiment.
To understand more about direct vs. indirect detection, including benefits of a secondary antibody and when to use one, please read our resources on Direct vs. Indirect Detection Methods.
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | BAF018 | |||
| HRP (Horseradish Peroxidase) | HAF018 | |||
| NL493 | NL009 | |||
| NL637 | NL008 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | HAF018 | |||
| Carrier Free | D-201-C-ABS2 | |||
| Carrier Free | NL008 | |||
| Carrier Free | NL009 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | D-301-C-ABS2 | |||
| Carrier Free | NL004 | |||
| Carrier Free | NL005 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-75281 | |||
| DyLight 350 | NBP1-75635 | |||
| DyLight 488 | NBP1-75285 | |||
| DyLight 550 | NBP1-75636 | |||
| DyLight 594 | NBP1-75637 | |||
| DyLight 633 | NBP1-75638 | |||
| FITC | NBP1-75282 | |||
| HRP | NBP1-75283 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75281 | |||
| Pre-adsorbed | NBP1-75282 | |||
| Pre-adsorbed | NBP1-75283 | |||
| Pre-adsorbed | NBP1-75285 | |||
| Pre-adsorbed | NBP1-75635 | |||
| Pre-adsorbed | NBP1-75636 | |||
| Pre-adsorbed | NBP1-75637 | |||
| Pre-adsorbed | NBP1-75638 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB7355 | |||
| Allophycocyanin | NB7354APC | |||
| Biotin | NB7354B | |||
| DyLight 350 | NB7354UV | |||
| DyLight 405 | NB7354V | |||
| DyLight 488 | NB7354G | |||
| DyLight 550 | NB7354R | |||
| DyLight 594 | NB7354DL594 | |||
| DyLight 650 | NB7354C | |||
| DyLight 680 | NB7354FR | |||
| DyLight 755 | NB7354IR | |||
| FITC | NB7356 | |||
| HRP | NB7357 | |||
| PE | NB7354PE | |||
| PerCP | NB7354PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | F0108 | |||
| Biotin | BAF109 | |||
| Fluorescein | F0109 | |||
| HRP (Horseradish Peroxidase) | HAF109 | |||
| HRP Polymer | VC004-025 | |||
| HRP Polymer | VC004-050 | |||
| HRP Polymer | VC004-125 | |||
| HRP Polymer | VC004-250 | |||
| NL493 | NL003 | |||
| NL557 | NL001 | |||
| NL637 | NL002 | |||
| PerCP (Peridinin-chlorophyll Protein Complex) | F0124 | |||
| Phycoerythrin | F0107 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | F0108 | |||
| Carrier Free | HAF109 | |||
| Carrier Free | F0107 | |||
| Carrier Free | F0109 | |||
| Carrier Free | F0124 | |||
| Carrier Free | NL001 | |||
| Carrier Free | NL002 | |||
| Carrier Free | NL003 | |||
| Carrier Free | VC004-025 | |||
| Carrier Free | VC004-050 | |||
| Carrier Free | VC004-125 | |||
| Carrier Free | VC004-250 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-74819 | |||
| Biotin | NBP1-74820 | |||
| DyLight 488 | NBP1-74824 | |||
| DyLight 550 | NBP1-75606 | |||
| DyLight 594 | NBP1-75607 | |||
| DyLight 650 | NBP1-75609 | |||
| FITC | NBP1-74821 | |||
| HRP | NBP1-74822 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-74819 | |||
| Pre-adsorbed | NBP1-74820 | |||
| Pre-adsorbed | NBP1-74821 | |||
| Pre-adsorbed | NBP1-74822 | |||
| Pre-adsorbed | NBP1-74824 | |||
| Pre-adsorbed | NBP1-75606 | |||
| Pre-adsorbed | NBP1-75607 | |||
| Pre-adsorbed | NBP1-75609 | |||
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0012.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows detection of PD-1 in mouse frozen tissue sections (salivary gland injected with human T cells) using DyLight™ 488 conjugated donkey anti-goat IgG (H+L) secondary antibody (green). Image from verified customer review. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0007.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None. Image using the Texas Red form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0001.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG, and DyLight 649 Donkey anti-Goat IgG at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol and dried. Image using the DyLight 649 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows anti-Histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP2-60657-img0013.jpg)
![ELISA: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - ELISA Results of Donkey Donkey anti-Goat IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 ug of Goat IgG as well as Chicken IgG, Guinea Porcine IgG, Hamster IgG, Equine IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution is 1:56,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG Mx7 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP2-60657-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75279-img0006.jpg "Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 form of this antibody.")
![Immunohistochemistry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of mouse tissue. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-NBP1-75279-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of human pancreatic cancer cells. Note: Need to be careful about bleeding into different filters since it might interfere with FITC. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75279-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of human osteosarcoma cell line. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75279-img0005.jpg)
![Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75286-img0009.jpg "Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of human osteosarcoma cell using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75286-img0006.jpg)
![Immunohistochemistry-Frozen: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of frozen mouse tissue using DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Frozen-NBP1-75286-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - analysis of LM7 cells using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to DyLight 594. Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75286-img0007.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - Analysis using Donkey anti-Goat IgG (H+L) Secondary Antibody using Embryo. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Western-Blot-NB7354-img0005.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - Analysis using Donkey anti-Goat IgG (H+L) Secondary Antibody using Embryo. Image using the HRP form of this antibody.")
![Immunohistochemistry-Paraffin: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:5,000 (0.2ug/ml). Secondary Antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody HRP Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Immunohistochemistry-Paraffin-NB7354-img0004.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image using the Fluorescein form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6707-img0009.jpg "Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image using the Fluorescein form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Immunocytochemistry/Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72930]](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB120-6707-img0011.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary Antibody: None. Secondary antibody: Mouse IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Other Band(s): None. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6707-img0003.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image using the Texas Red form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6707-img0005.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - DyLight (R) dyes can be used for two-color western blot detection with low background and high signal. Image using the DyLight 680 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6707-img0008.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-6707-img0016.jpg)
![ELISA: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - ELISA results of purified Donkey anti-Mouse IgG (H+L) Secondary Antibody [Biotin] tested against Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG (purple line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer, Streptavidin HRP conjugate, and TMB substrate.. Using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-ELISA-NB120-6707-img0017.jpg)
![ELISA: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - ELISA Results of Donkey anti-Mouse IgG (H+L) Secondary antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG. The working dilution is 1:128,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer , Goat Anti-Donkey HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-ELISA-NB120-6707-img0018.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74818] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat anti-P-cadherin polyclonal antibody (AF761) followed by a 1:5000 Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to HRP (NBP1-74822). Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-74818-img0004.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74818] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat anti-P-cadherin polyclonal antibody (AF761) followed by a 1:5000 Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to HRP (NBP1-74822). Image using the HRP form of this antibody.")
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74818] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat anti-P-cadherin polyclonal antibody (AF761) followed by a 1:5000 donkey anti-goat IgG-HRP (NBP1-74822). Image using the HRP form of Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-74818-img0005.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Western Blot of Donkey anti-Goat IgG antibody. Lane 1: Goat IgG. Load: 50 ng per lane. Primary antibody: NBP2-60656 incubated overnight at 4C. Secondary antibody: Peroxidase donkey secondary antibody incubated for 30 min at RT; incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 and 55 kDa for Goat IgG.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Western-Blot-NBP2-60656-img0009.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Western Blot of Donkey anti-Goat IgG antibody. Lane 1: Goat IgG. Load: 50 ng per lane. Primary antibody: NBP2-60656 incubated overnight at 4C. Secondary antibody: Peroxidase donkey secondary antibody incubated for 30 min at RT; incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 and 55 kDa for Goat IgG.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60656-img0016.jpg)
![Dot Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Antigen: Goat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Donkey anti-Goat IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT. Image from the Alkaline Phosphatase version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Dot-Blot-NBP2-60656-img0010.jpg)
![Dot Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Antigen: Goat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Donkey anti-Goat IgG Antibody Texas Red Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT. Image from the Texas Red version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Dot-Blot-NBP2-60656-img0011.jpg)
![ELISA: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - ELISA results of purified Donkey anti-Goat IgG antibody Biotin conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 ug of Goat IgG. The starting dilution of antibody was 5 ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer, and TMB substrate.. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-ELISA-NBP2-60656-img0013.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60656-img0017.jpg)
