Novus Biologicals products are now on bio-techne.com

Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

Images

 
Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Polyclonal Antibody (Catalog # AF3584) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This ...read more
Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP (Horseradish Peroxidase)

Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

Immunogen
Goat IgG
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Simple Western
  • Western Blot
Publications
Read Publications using
HAF109 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a 0.2 um filtered solution in PBS with stabilizers.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-62076
Species: Hu
Applications: ELISA, ELISA(Cap), ELISA(Det)
NBP1-74190
Species: Mu
Applications: ChIP, WB
NBP2-79716
Species: Hu
Applications: IHC, IHC-P, PA
NB7276
Species: Ch
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-30955
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
H00728695-B01P
Species: Hu
Applications: ICC/IF, IP, WB
NBP1-88927
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-29373
Species: Hu, Mu, Rt
Applications: Flow-CS, Flow, ICC/IF
NBP2-43666
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87404
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
NBP2-62025
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, ELISA(Cap), ELISA(Det)
DHAPG0
Species: Hu
Applications: ELISA
NBP1-43435
Species: Hu, Mu
Applications: Flow, WB
NBP2-19784
Species: Hu, Mu, Rt, Ye
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, PLA, WB
AF8537
Species: Mu, Rt
Applications: ICC, Simple Western, WB
AF1938
Species: Hu
Applications: ICC, IP, Simple Western, WB

Publications for IgG Antibody (HAF109)(127)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Bacteria, Bovine, Canine, Goat, N/A, Virus.

We have publications tested in 11 applications: Bioassay, Co-Immunoprecipitation, ELISA Detection, ELISA Development, Flow Cytometry, IHC, IHC-Fr, Secondary Antibody, Simple Western, Western Blot, Western Blots.


Filter By Application
Bioassay
(1)
Co-Immunoprecipitation
(1)
ELISA Detection
(2)
ELISA Development
(2)
Flow Cytometry
(1)
IHC
(1)
IHC-Fr
(1)
Secondary Antibody
(1)
Simple Western
(1)
Western Blot
(42)
Western Blots
(1)
All Applications
Filter By Species
Human
(29)
Mouse
(17)
Rat
(3)
Bacteria
(1)
Bovine
(1)
Canine
(1)
Goat
(1)
N/A
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 127. Show All 127 Publications.
Publications using HAF109 Applications Species
Bhattacharya, R;Ward, T;Kalejaiye, TD;Mishra, A;Leeman, S;Arzaghi, H;Seidman, JG;Seidman, CE;Musah, S; Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39211233] (Western Blot, Human) Western Blot Human
Fujii, K;Fujishima, Y;Kita, S;Kawada, K;Fukuoka, K;Sakaue, TA;Okita, T;Kawada-Horitani, E;Nagao, H;Fukuda, S;Maeda, N;Nishizawa, H;Shimomura, I; Pharmacological HIF-1 activation upregulates extracellular vesicle production synergistically with adiponectin through transcriptional induction and protein stabilization of T-cadherin Scientific reports 2024-02-13 [PMID: 38351156] (Western Blot, Mouse) Western Blot Mouse
Martin Flores, N;Podpolny, M;McLeod, F;Workman, I;Crawford, K;Ivanov, D;Leonenko, G;Escott-Price, V;Salinas, PC; Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer's disease, restores synapse integrity and memory in a disease mouse model eLife 2024-01-29 [PMID: 38285009] (Western Blot, Human) Western Blot Human
Rybakova, EY;Avdonin, PP;Trufanov, SK;Goncharov, NV;Avdonin, PV; Synergistic Interaction of 5-HT1B and 5-HT2B Receptors in Cytoplasmic Ca2+ Regulation in Human Umbilical Vein Endothelial Cells: Possible Involvement in Pathologies International journal of molecular sciences 2023-09-08 [PMID: 37762136] (Western Blot, Human) Western Blot Human
Shrestha, M;Wang, DY;Ben-David, Y;Zacksenhaus, E; CDK4/6 inhibitors and the pRB-E2F1 axis suppress PVR and PD-L1 expression in triple-negative breast cancer Oncogenesis 2023-05-26 [PMID: 37230983] (Western Blot, Mouse) Western Blot Mouse
L Wiedmann, F De Angelis, N Vaquero-Si, E Donato, E Espinet, I Moll, E Alsina-San, H Bohnenberg, E Fernandez-, R Mülfarth, M Vacca, J Gerwing, LC Conradi, P Ströbel, A Trumpp, C Mogler, A Fischer, J Rodriguez- HAPLN1 potentiates peritoneal metastasis in pancreatic cancer Nature Communications, 2023-04-24;14(1):2353. 2023-04-24 [PMID: 37095087] (Western Blot, Goat) Western Blot Goat
Alvin Phan, Hugo Avila, J. Andrew MacKay Biomimetic SARS-CoV-2 Spike Protein Nanoparticles Biomacromolecules 5/8/2023 [PMID: 37001147]
KE Stephenson, J Porte, A Kelly, WA Wallace, CE Huntington, CL Overed-Say, ES Cohen, RG Jenkins, AE John The IL-33:ST2 axis is unlikely to play a central fibrogenic role in idiopathic pulmonary fibrosis Respiratory Research, 2023-03-23;24(1):89. 3/23/2023 [PMID: 36949463]
Pauline P. Marie, Shih‐Jung Fan, John Mason, Adam Wells, Cláudia C. Mendes, S. Mark Wainwright et al. Accessory ESCRT‐III proteins are conserved and selective regulators of Rab11a‐exosome formation Journal of Extracellular Vesicles 3/5/2023 [PMID: 36872252]
Hiroyuki Suzuki, Hideki Iwamoto, Takahiro Seki, Toru Nakamura, Atsutaka Masuda, Takahiko Sakaue et al. Tumor‐derived insulin‐like growth factor‐binding protein‐1 contributes to resistance of hepatocellular carcinoma to tyrosine kinase inhibitors Cancer Communications 4/1/2023 [PMID: 36825684]
Show All 127 Publications.

Reviews for IgG Antibody (HAF109) (0)

There are no reviews for IgG Antibody (HAF109). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Antibody (HAF109) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Annexin V Apoptosis Kit [FITC]
NBP2-29373-100Tests

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] and receive a gift card or discount.

Bioinformatics

Gene Symbol IGHG1