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Donkey anti-Goat IgG Secondary Antibody [NL493]

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Detection of human EGFR by incubating cells with 5 μg/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
NL493

Donkey anti-Goat IgG Secondary Antibody [NL493] Summary

Additional Information
NorthernLights 493 Fluorochrome-labeled Antibody, X Absorbed
Immunogen
Goat IgG
Specificity
Goat IgG (H+L)
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunocytochemistry
Reviewed Applications
Read 1 Review rated 5
using
NL003 in the following application:

Publications
Read Publications using
NL003 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [NL493]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (NL003)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC, Immunocytochemistry.


Filter By Application
Flow Cytometry
(1)
ICC
(6)
IHC
(3)
Immunocytochemistry
(2)
All Applications
Filter By Species
Human
(7)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NL003 Applications Species
Tonin, R;Feo, F;Falliano, S;Giunti, L;Calamai, M;Procopio, E;Mari, F;Sciruicchio, V;Conti, V;Fanelli, I;Bambi, F;Guerrini, R;Morrone, A; Generation of a human induced pluripotent stem cell line from a patient with GM3 synthase deficiency using self-replicating RNA vector Stem cell research 2024-05-03 [PMID: 38703669] (Immunocytochemistry, Human) Immunocytochemistry Human
Rafii, P;Cruz, PR;Ettich, J;Seibel, C;Padrini, G;Wittich, C;Lang, A;Petzsch, P;Köhrer, K;Moll, JM;Floss, DM;Scheller, J; Engineered Interleukin-6-derived cytokines recruit artificial receptor complexes and disclose CNTF signaling via the OSMR The Journal of biological chemistry 2024-04-01 [PMID: 38569939] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Meas, S;Chaimongkolnukul, K;Narkpuk, J;Mekvichitsaeng, P;Poomputsa, K;Wanasen, N;Roshorm, YM; Humoral and Cellular Immune Responses Induced by Bivalent DNA Vaccines Expressing Fusion Capsid Proteins of Porcine Circovirus Genotypes 2a and 2b Vaccines 2024-03-18 [PMID: 38543958] (Immunocytochemistry, Human) Immunocytochemistry Human
Sweeney, A;Arora, A;Edwards, S;Mallidi, S; Ultrasound-guided Photoacoustic image Annotation Toolkit in MATLAB (PHANTOM) for preclinical applications bioRxiv : the preprint server for biology 2023-11-10 [PMID: 37986998] (IHC, Mouse) IHC Mouse
Julia Ettich, Julia Werner, Hendrik T. Weitz, Eva Mueller, Roland Schwarzer, Philipp A. Lang et al. A Hybrid Soluble gp130/Spike-Nanobody Fusion Protein Simultaneously Blocks Interleukin-6 trans -Signaling and Cellular Infection with SARS-CoV-2 Journal of Virology 2022-02-23 [PMID: 34935434]
P Ghasemi-De, A Doosti, MS Jami The functions of azurin of Pseudomonas aeruginosa and human mammaglobin-A on proapoptotic and cell cycle regulatory genes expression in the MCF-7 breast cancer cell line Saudi journal of biological sciences, 2020-04-14;27(9):2308-2317. 2020-04-14 [PMID: 32884412] (ICC, Human) ICC Human
Ameneh Mehri Ghahfarrokhi, Mohammad Saeid Jami, Morteza Hashemzadeh Chaleshtori Upregulation of Neuroprogenitor and Neural Markers via Enforced miR-124 and Growth Factor Treatment Int J Mol Cell Med 2020-08-25 [PMID: 32832485]
C Miyagi-Shi, Y Nakashima, N Kobayashi, I Saitoh, M Watanabe, H Noguchi Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA Sci Rep, 2018-08-17;8(1):12341. 2018-08-17 [PMID: 30120295] (ICC, Mouse) ICC Mouse
Hai Li, Dahang Zhao, Shengjing Wang, Jing Ding, Li Zhao Bone morphogenetic protein-9 promotes the differentiation of mouse spleen macrophages into osteoclasts via the ALK1 receptor and ERK 1/2 pathways in vitro Molecular Medicine Reports 2016-11-01 [PMID: 27748860]
I Kryczek, L Wang, K Wu, W Li, E Zhao, T Cui, S Wei, Y Liu, Y Wang, L Vatan, W Szeliga, JK Greenson, J Roli?ski, W Zgodzinski, E Huang, K Tao, G Wang, W Zou Inflammatory regulatory T cells in the microenvironments of ulcerative colitis and colon carcinoma Oncoimmunology, 2016-08-12;5(8):e1105430. 2016-08-12 [PMID: 27622054] (IHC, Human) IHC Human
Show All 16 Publications.

Review for IgG Antibody (NL003) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NL003:
Filter by Applications
IHC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry IgG NL003
Enlarge
5
reviewed by:
Hayk Simonyan
IHC Mouse 06/23/2017
View

Summary

ApplicationImmunohistochemistry
Sample TestedMouse cortex
SpeciesMouse

FAQs for IgG Antibody (NL003) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

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1
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Hayk Simonyan
06/23/2017
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol IGHG1