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Donkey anti-Goat IgG Secondary Antibody [Phycoerythrin]

Images

 
SH-SY5Y human neuroblastoma cell line was stained with GoatAnti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody(Catalog # AF496,filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Donkey
Conjugate
Phycoerythrin

Donkey anti-Goat IgG Secondary Antibody [Phycoerythrin] Summary

Immunogen
Goat IgG
Specificity
Goat IgG specific IgG was purified by goat IgG (H+L) affinity chromatography.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 1 Review rated 5
using
F0107 in the following application:

Publications
Read Publications using
F0107 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [Phycoerythrin]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (F0107)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Flow Cytometry, ICC, Secondary Antibody.


Filter By Application
Flow Cytometry
(9)
ICC
(1)
Secondary Antibody
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using F0107 Applications Species
Kummarapurugu, AB;Hawkridge, AM;Ma, J;Osei, S;Martin, RK;Zheng, S;Voynow, JA; Neutrophil Elastase decreases SARS-CoV-2 Spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomian from the epithelial surface The Journal of biological chemistry 2023-05-13 [PMID: 37187291] (Flow Cytometry, Human) Flow Cytometry Human
JS Mytych, Z Pan, C Lopez-Davi, N Redinger, C Lawrence, J Ziegler, N Popescu, JA James, AD Farris Peptidoglycan from Bacillus anthracis Inhibits Human Macrophage Efferocytosis in Part by Reducing Cell Surface Expression of MERTK and TIM-3 bioRxiv : the preprint server for biology, 2023-05-10;0(0):. 5/10/2023 [PMID: 37066181]
Z Awida, S Hiram-Bab, A Bachar, H Saed, D Zyc, A Gorodov, N Ben-Califa, S Omari, J Omar, L Younis, JA Iden, L Graniewitz, I Gluzman, T Liron, B Raphael-Mi, A Kolomansky, M Rauner, B Wielockx, Y Gabet, D Neumann Erythropoietin Receptor (EPOR) Signaling in the Osteoclast Lineage Contributes to EPO-Induced Bone Loss in Mice International Journal of Molecular Sciences, 2022-10-10;23(19):. 2022-10-10 [PMID: 36233351] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Youn SE, Jiang F, Won HY et al. PAUF Induces Migration of Human Pancreatic Cancer Cells Exclusively via the TLR4/MyD88/NF-kappa B Signaling Pathway International journal of molecular sciences 9/27/2022 [PMID: 36232715]
Johannes Höfle, Timo Trenkner, Nadja Kleist, Vera Schwane, Sarah Vollmers, Bryan Barcelona et al. Engagement of TRAIL triggers degranulation and IFN gamma production in human natural killer cells EMBO reports 8/3/2022 [PMID: 35758160]
J Grau-Expós, D Perea, M Suppi, N Massana, A Vergara, MJ Soler, B Trinite, J Blanco, J García-Pér, J Alcamí, A Serrano-Mo, J Rosado, V Falcó, M Genescà, MJ Buzon Evaluation of SARS-CoV-2 entry, inflammation and new therapeutics in human lung tissue cells PloS Pathogens, 2022-01-13;18(1):e1010171. 2022-01-13 [PMID: 35025963] (ICC, Human) ICC Human
Z Awida, A Bachar, H Saed, A Gorodov, N Ben-Califa, M Ibrahim, A Kolomansky, JA Iden, L Graniewitz, T Liron, S Hiram-Bab, M Brines, Y Gabet, D Neumann The Non-Erythropoietic EPO Analogue Cibinetide Inhibits Osteoclastogenesis In Vitro and Increases Bone Mineral Density in Mice International Journal of Molecular Sciences, 2021-12-21;23(1):. 2021-12-21 [PMID: 35008482] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Courtney M. Smith, Alice Li, Nithya Krishnamurthy, Mark A. Lemmon Phosphatidylserine binding directly regulates TIM-3 function Biochemical Journal 9/17/2021 [PMID: 34435619]
X Yuan, N Qian, S Ling, Y Li, W Sun, J Li, R Du, G Zhong, C Liu, G Yu, D Cao, Z Liu, Y Wang, Z Qi, Y Yao, F Wang, J Liu, S Hao, X Jin, Y Zhao, J Xue, D Zhao, X Gao, S Liang, Y Li, J Song, S Yu, Y Li Breast cancer exosomes contribute to pre-metastatic niche formation and promote bone metastasis of tumor cells Theranostics, 2021-01-01;11(3):1429-1445. 2021-01-01 [PMID: 33391543] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Agnès Quéméner, Sébastien Morisseau, Rui P. Sousa, Kilian Trillet, Mike Maillasson, Isabelle Leray et al. IL-15R alpha membrane anchorage in either cis or trans is required for stabilization of IL-15 and optimal signaling Journal of Cell Science 3/1/2020 [PMID: 31653781]
Show All 19 Publications.

Review for IgG Antibody (F0107) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using F0107:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry IgG F0107
Enlarge
5
reviewed by:
Verified Customer
Flow Human 04/17/2017
View

Summary

ApplicationFlow Cytometry
Sample TestedMCF-7 human breast cancer cell line
SpeciesHuman
LotLWW0616051

Comments

CommentsFlow cytometry analysis of MCF-7 cells stained with Goat anti-Human E-cadherin antibody (red) or Isotype Control IgG (black), followed by 10 uL/106 cells of PE-conjugated Donkey Anti-Goat secondary antibody (#F0107).

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IgG Antibody (F0107) (0)

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Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

5
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Verified Customer
04/17/2017
Application: Flow
Species: Human

Bioinformatics

Gene Symbol IGHG1