Novus Biologicals products are now on bio-techne.com

Donkey anti-Goat IgG Secondary Antibody [Unconjugated]

Images

 
There are currently no images for IgG Antibody (AF109).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Donkey
Conjugate
Unconjugated
Concentration
LYOPH

Donkey anti-Goat IgG Secondary Antibody [Unconjugated] Summary

Immunogen
Goat IgG
Specificity
Detects goat IgG heavy and light chains. In direct ELISAs, approximately 5% cross-reactivity with human IgG, mouse IgG, rabbit IgG and less than 1% cross-reactivity with chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 dilution
Publications
Read Publications using
AF109 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [Unconjugated]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-62076
Species: Hu
Applications: ELISA, ELISA(Cap), ELISA(Det)
NBP1-74190
Species: Mu
Applications: ChIP, WB
NBP2-79716
Species: Hu
Applications: IHC, IHC-P, PA
NB7276
Species: Ch
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-30955
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
H00728695-B01P
Species: Hu
Applications: ICC/IF, IP, WB
NBP1-88927
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-29373
Species: Hu, Mu, Rt
Applications: Flow-CS, Flow, ICC/IF
NBP2-43666
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87404
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
NBP2-62025
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, ELISA(Cap), ELISA(Det)
DHAPG0
Species: Hu
Applications: ELISA
NBP1-43435
Species: Hu, Mu
Applications: Flow, WB
NBP2-19784
Species: Hu, Mu, Rt, Ye
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, PLA, WB
AF8537
Species: Mu, Rt
Applications: ICC, Simple Western, WB
AF1938
Species: Hu
Applications: ICC, IP, Simple Western, WB

Publications for IgG Antibody (AF109)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Control, Dot Blot, Flow Cytometry, IHC-P, Western Blot.


Filter By Application
Control
(2)
Dot Blot
(1)
Flow Cytometry
(1)
IHC-P
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(4)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF109 Applications Species
N Kurgan, B Baranowski, J Stoikos, AJ MacNeil, VA Fajardo, REK MacPherson, P Klentrou Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male mice Frontiers in Physiology, 2023-01-04;13(0):1061715. 2023-01-04 [PMID: 36685192] (Western Blot, Mouse) Western Blot Mouse
Dan Hu, Ruo Yu Meng, Thi Van Nguyen, Ok Hee Chai, Byung Hyun Park, Ju-Seog Lee et al. Inhibition of colorectal cancer tumorigenesis by ursolic acid and doxorubicin is mediated by targeting the Akt signaling pathway and activating the Hippo signaling pathway Molecular Medicine Reports 11/15/2022 [PMID: 36382656]
W Mori, K Yuzu, N Lobsiger, H Nishioka, H Sato, T Nagase, K Iwaya, M Lindgren, T Zako Degradation of insulin amyloid by antibiotic minocycline and formation of toxic intermediates Scientific Reports, 2021-03-25;11(1):6857. 2021-03-25 [PMID: 33767265] (Dot Blot, Human) Dot Blot Human
H Imamura, D Nagayama, N Ishihara, S Tanaka, R Watanabe, Y Watanabe, Y Sato, T Yamaguchi, N Ban, H Kawana, M Ohira, K Endo, A Saiki, K Shirai, I Tatsuno Resveratrol attenuates triglyceride accumulation associated with upregulation of Sirt1 and lipoprotein lipase in 3T3-L1 adipocytes Mol Genet Metab Rep, 2017-05-30;12(0):44-50. 2017-05-30 [PMID: 28580300] (Western Blot, Mouse) Western Blot Mouse
Hussain , Sajid, Rodriguez-Fernandez , Maria, Braun , Gary B, Doyle , Francis, Ruoslahti , Erkki Quantity and accessibility for specific targeting of receptors in tumours. Sci Rep, 2014-06-10;4(0):5232. 2014-06-10 [PMID: 24912981] (Human) Human
Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening. Bone, 2006-12-21;40(5):1276-83. 2006-12-21 [PMID: 17236833] (Control, Mouse) Control Mouse
Cross SS, Yang Z, Brown NJ, Balasubramanian SP, Evans CA, Woodward JK, Neville-Webbe HL, Lippitt JM, Reed MW, Coleman RE, Holen I Osteoprotegerin (OPG)--a potential new role in the regulation of endothelial cell phenotype and tumour angiogenesis? Int. J. Cancer, 2006-04-15;118(8):1901-8. 2006-04-15 [PMID: 16287088] (Flow Cytometry, Human) Flow Cytometry Human
Latchney LR, Fallon MA, Culp DJ, Gelbard HA, Dewhurst S Immunohistochemical assessment of fractalkine, inflammatory cells, and human herpesvirus 7 in human salivary glands. J. Histochem. Cytochem., 2004-05-01;52(5):671-81. 2004-05-01 [PMID: 15100244] (IHC-P, Human) IHC-P Human
Ronchetti S, Zollo O, Bruscoli S, Agostini M, Bianchini R, Nocentini G, Ayroldi E, Riccardi C GITR, a member of the TNF receptor superfamily, is costimulatory to mouse T lymphocyte subpopulations. Eur. J. Immunol., 2004-03-01;34(3):613-22. 2004-03-01 [PMID: 14991590] (Control, Mouse) Control Mouse

Reviews for IgG Antibody (AF109) (0)

There are no reviews for IgG Antibody (AF109). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Antibody (AF109) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Annexin V Apoptosis Kit [FITC]
NBP2-29373-100Tests

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Donkey anti-Goat IgG Secondary Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol IGHG1