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Donkey anti-Rabbit IgG Secondary Antibody [NL493]

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Detection of human Ki-67 by incubating cells with 3 μg/mL of Rabbit anti-Human Ki-67 monoclonal antibody (MAB7617) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rabbit IgG ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
NL493

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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Donkey anti-Rabbit IgG Secondary Antibody [NL493] Summary

Additional Information
NorthernLights 493 Fluorochrome-labeled Antibody, X Absorbed
Immunogen
Rabbit IgG
Specificity
Rabbit IgG (H+L)
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunocytochemistry
Publications
Read Publications using
NL006 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Rabbit IgG Secondary Antibody [NL493]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (NL006)(14)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Cercopithecus aethiops.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunohistochemistry.


Filter By Application
Flow Cytometry
(1)
ICC
(3)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
Immunohistochemistry
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Rat
(1)
Bovine
(1)
Cercopithecus aethiops
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NL006 Applications Species
Berling, FP;Mendes, CM;Goissis, MD; Influence of glucose and oxygen tension on the trophectoderm and the inner cell mass of in vitro produced bovine embryos Theriogenology 2024-05-23 [PMID: 38796961] (Immunohistochemistry, Bovine) Immunohistochemistry Bovine
C Geller, J Maddela, R Tuplano, F Runa, Y Adamian, R Güth, GO Soto, L Tomaneng, J Cantor, JA Kelber Fibronectin, DHPS and SLC3A2 Signaling Cooperate to Control Tumor Spheroid Growth, Subcellular eIF5A1/2 Distribution and CDK4/6 Inhibitor Resistance bioRxiv : the preprint server for biology, 2023-04-16;0(0):. 2023-04-16 [PMID: 37090582]
Susanne N. Wijesinghe, Amel Badoume, Dominika E. Nanus, Archana Sharma‐Oates, Hussein Farah, Michelangelo Certo et al. Obesity defined molecular endotypes in the synovium of patients with osteoarthritis provides a rationale for therapeutic targeting of fibroblast subsets Clinical and Translational Medicine 2023-04-03 [PMID: 37006170]
Valeria Fernández Vallone, Francisco Raúl Borzone, Leandro Marcelo Martinez, María Belén Giorello, Hosoon Choi, Federico Dimase et al. Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients Frontiers in Oncology 2023-02-20 [PMID: 36890825]
DA Clark, A Reihani, JL Arredondo, K Ask, WG Foster CD200S-positive granulated lymphoid cells in endometrium appear to be CD56-positive uterine NK cells Journal of reproductive immunology, 2022-01-14;150(0):103477. 2022-01-14 [PMID: 35051828] (IHC, Human) IHC Human
MS Szymczak, WK Reeves, MM Miller Further Characterization of Rio Grande Virus and Potential for Cross Reactivity with Rift Valley Fever Virus Assays Viruses, 2021-08-30;13(9):. 2021-08-30 [PMID: 34578299] (ICC, Cercopithecus aethiops) ICC Cercopithecus aethiops
T Liu, F Gonzalez D, Y Zhao, Z Wu, AE Rinke, KK Kim, SH Phan Telomerase reverse transcriptase ameliorates lung fibrosis by protecting alveolar epithelial cells against senescence J. Biol. Chem., 2019-04-18;0(0):. 2019-04-18 [PMID: 31000627] (IHC-P, Mouse) IHC-P Mouse
EO Farombi, IO Awogbindin, TH Farombi, JO Oladele, ER Izomoh, OB Aladelokun, IO Ezekiel, OI Adebambo, VO Abah Neuroprotective role of kolaviron in striatal redo-inflammation associated with rotenone model of Parkinson&#039;s disease Neurotoxicology, 2019-03-28;73(0):132-141. 2019-03-28 [PMID: 30930291] (IHC, Rat) IHC Rat
The Dual NOD1/NOD2 Agonism of Muropeptides Containing a Meso-Diaminopimelic Acid Residue PLoS ONE, 2016-08-11;11(8):e0160784. 2016-08-11 [PMID: 27513337] (Flow Cytometry) Flow Cytometry
Daniel Patschan, Katrin Schwarze, Elvira Henze, Susann Patschan, Gerhard Anton Müller Endothelial autophagy and Endothelial-to-Mesenchymal Transition (EndoMT) in eEPC treatment of ischemic AKI Journal of Nephrology 2016-10-01 [PMID: 26289253]
Show All 14 Publications.

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Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol IGHG1