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Donkey anti-Mouse IgG Secondary Antibody [NL637]

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Detection of human Vimentin by incubating cells with 8 μg/mL of Mouse anti-Human Vimentin monoclonal antibody (MAB21052) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
NL637

Order Details

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View Available Formulations
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Donkey anti-Mouse IgG Secondary Antibody [NL637] Summary

Additional Information
NorthernLights 637 Fluorochrome-labeled Antibody, X Absorbed
Immunogen
Mouse IgG
Specificity
Mouse IgG (H+L)
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
Publications
Read Publications using
NL008 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Mouse IgG Secondary Antibody [NL637]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (NL008)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Chicken, Transgenic Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P.


Filter By Application
Flow Cytometry
(1)
ICC
(2)
IHC
(2)
IHC-Fr
(5)
IHC-P
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(4)
Chicken
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NL008 Applications Species
D Halperin, A Stavsky, R Kadir, M Drabkin, O Wormser, Y Yogev, V Dolgin, R Proskorovs, Y Perez, H Nudelman, O Stoler, B Rotblat, T Lifschytz, A Lotan, G Meiri, D Gitler, OS Birk CDH2 mutation affecting N-cadherin function causes attention-deficit hyperactivity disorder in humans and mice Nature Communications, 2021-10-26;12(1):6187. 2021-10-26 [PMID: 34702855] (ICC, Mouse, Transgenic Mouse) ICC Mouse, Transgenic Mouse
C Salesse, J Charest, H Doucet-Bea, AM Castonguay, S Labrecque, P De Koninck, M Lévesque Opposite Control of Excitatory and Inhibitory Synapse Formation by Slitrk2 and Slitrk5 on Dopamine Neurons Modulates Hyperactivity Behavior Cell Rep, 2020-02-18;30(7):2374-2386.e5. 2020-02-18 [PMID: 32075770] (ICC, Mouse) ICC Mouse
Anna Kreutz, Nicole Barger Maximizing Explanatory Power in Stereological Data Collection: A Protocol for Reliably Integrating Optical Fractionator and Multiple Immunofluorescence Techniques Frontiers in Neuroanatomy 2018-10-30 [PMID: 30425623]
Szaloki N, Krieger J, Komaromi I, Toth K, Vamosi G Evidence for Homodimerization of the c-Fos Transcription Factor in Live Cells Revealed by Fluorescence Microscopy and Computer Modeling. Mol Cell Biol, 2015-08-24;35(21):3785-98. 2015-08-24 [PMID: 26303532] (IHC, Human) IHC Human
Kopp T, Riedl E, Bangert C, Bowman E, Greisenegger E, Horowitz A, Kittler H, Blumenschein W, McClanahan T, Marbury T, Zachariae C, Xu D, Hou X, Mehta A, Zandvliet A, Montgomery D, van Aarle F, Khalilieh S Clinical improvement in psoriasis with specific targeting of interleukin-23. Nature, 2015-03-09;521(7551):222-6. 2015-03-09 [PMID: 25754330] (IHC, Human) IHC Human
Shulman Y, Stavsky A, Fedorova T, Mikulincer D, Atias M, Radinsky I, Kahn J, Slutsky I, Gitler D ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons. J Neurosci, 2015-01-21;35(3):985-98. 2015-01-21 [PMID: 25609616] (IHC-Fr, Mouse) IHC-Fr Mouse
Anusuya Das, Steven M. Lenz, Anthony O. Awojoodu, Edward A. Botchwey Abluminal Stimulation of Sphingosine 1-Phosphate Receptors 1 and 3 Promotes and Stabilizes Endothelial Sprout Formation Tissue Engineering Part A 2015-01-01 [PMID: 25315888]
Jianchao Ma, Bin Zhang, Shuangxin Liu, Shaoting Xie, Yun Yang, Juan Ma et al. 1,25-Dihydroxyvitamin D(3) Inhibits Podocyte uPAR Expression and Reduces Proteinuria PLoS ONE 2013-05-31 [PMID: 23741418]
Bin Zhang, Wei Shi, Juan Ma, Alexis Sloan, Christian Faul, Changli Wei et al. The calcineurin–NFAT pathway allows for urokinase receptor-mediated beta3 integrin signaling to cause podocyte injury Journal of Molecular Medicine 2012-12-01 [PMID: 23015147]
Phenotypic characterization of leukocytes in prenatal human dermis. J. Invest. Dermatol., 2012-06-21;132(11):2581-92. 2012-06-21 [PMID: 22718119] (IHC-Fr, Human) IHC-Fr Human
Show All 15 Publications.

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Product General Protocols

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FAQs for IgG Antibody (NL008) (0)

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Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


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CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1