Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 applications: FLOW, Flow Cytometry, ICC/IF, IF/IHC.

Showing Publications 1 - 7 of 7.

Publications using NBP2-60657	Applications	Species
Starr R, Aguilar B, Gumber D et al. Inclusion of 4-1BB Costimulation Enhances Selectivity and Functionality of IL13R?2-Targeted Chimeric Antigen Receptor T Cells Cancer Research Communications 2023-01-17 [PMID: 36968221] (Flow Cytometry)	Flow Cytometry
Schlotter F, de Freitas RCC, Rogers MA Et al. ApoC-III is a novel inducer of calcification in human aortic valves J Biol Chem 2020-12-18 [PMID: 33334888] (FLOW) Details: Citation using the PE version of this antibody.	FLOW
Hamilton AM, Cheung WY, Gomez-Aristizabal A et al. Iron nanoparticle-labeled murine mesenchymal stromal cells in an osteoarthritic model persists and suggests anti-inflammatory mechanism of action PLoS One 2019-12-04 [PMID: 31794570] (IF/IHC, IF/IHC)	IF/IHC, IF/IHC
Agarwal R, Liu G, Tam NW et al. Precision cell delivery in biphasic polymer systems enhances growth of keratinocytes in culture and promotes their attachment on acellular dermal matrices J Tissue Eng Regen Med 2019-02-28 [PMID: 30811860] (ICC/IF)	ICC/IF
England CG, Jiang D, Ehlerding EB et al. (89)Zr-labeled nivolumab for imaging of T-cell infiltration in a humanized murine model of lung cancer Eur J Nucl Med Mol Imaging 2017-08-20 [PMID: 28821924] (IF/IHC)	IF/IHC
Mahadev V, Starr R, Wright SL et al. Cytokine Induction of VCAM-1 but Not IL13Ralpha2 on Glioma Cells: A Tale of Two Antibodies PLoS ONE 2014-05-05 [PMID: 24787244] (FLOW) Details: This reference used the PE version of NBP2-60657.	FLOW
Nishimura T, Hatoya S, Kanegi R et al. Generation of Functional Platelets from Canine Induced Pluripotent Stem Cells Stem Cells Dev 2013-02-14 [PMID: 23409943] Details: This reference used the PE version of NBP2-60657.

Array NBP2-60657

• IgG (H+L) Antibodies