Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - Analysis using Donkey anti-Goat IgG (H+L) Secondary Antibody using Embryo. Image using the HRP form of this antibody.
Immunohistochemistry-Paraffin: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:5,000 (0.2ug/ml). ...read more
This Donkey anti-Goat IgG (H+L) Secondary Antibody was developed against goat IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Donkey anti-Goat IgG (H+L) Secondary Antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified
Applications/Dilutions
Dilutions
Adhesion Activation
ELISA 1:1000 - 1:30000; for coating plates 1:100 - 1:500
Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).
The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).
3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268
4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Publications for IgG (H+L) Antibody (NB7354)(9)
We have publications tested in 2 applications: ELISA, WB.
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![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - Analysis using Donkey anti-Goat IgG (H+L) Secondary Antibody using Embryo. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Western-Blot-NB7354-img0005.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - Analysis using Donkey anti-Goat IgG (H+L) Secondary Antibody using Embryo. Image using the HRP form of this antibody.")
![Immunohistochemistry-Paraffin: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:5,000 (0.2ug/ml). Secondary Antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody HRP Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Immunohistochemistry-Paraffin-NB7354-img0004.jpg "Immunohistochemistry-Paraffin: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:5,000 (0.2ug/ml). Secondary Antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody HRP Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB Image using the HRP form of this antibody.")