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Histone H4 [p Ser1] Antibody - BSA Free

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Western Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - Analysis of Histone H4 pS1 in HeLa histone prep.

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, DB, ICC/IF, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.89 mg/ml

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Histone H4 [p Ser1] Antibody - BSA Free Summary

Immunogen
Synthetic phosphorylated peptide surrounding Serine 1 of human Histone H4 [Swiss Prot# P62805].
Modification
p Ser1
Localization
Nucleus.
Marker
H4pS1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H4C16
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 2-5 ug per million cells
  • Dot Blot 0.1 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:1000
Application Notes
This H4pS1 antibody is useful for ICC/IF, ChIP, Dot blot, and Western blot where a band is detected ~13 kDa.
Control Peptide
Histone H4 Peptide (NB21-2000PEP)
Publications
Read Publications using
NB21-2000 in the following applications:

Reactivity Notes

Predicted to react with most mammal species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.89 mg/ml
Purity
Immunogen affinity purified

Notes

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H4 [p Ser1] Antibody - BSA Free

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4M
  • H4S1p
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, phosphorylation of H4 Ser1 (H4 pS1) has been linked to mitosis and DNA repair. This modification is enriched in sites proximal to double stranded brakes, but not those associated with UV damage. Casein kinase II (CK2) phosphorylates H4 Ser1, and it also implicated in regulating the DNA damage response. Furthermore, recruitment of CK2 requires the SIN3/RPD3 histone deacetylase complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol H4C16
Entrez
Uniprot