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WNK3 Antibody - Azide and BSA Free

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Western Blot: WNK3 Antibody [NBP2-93100] - Analysis of HAP1, using WNK3 antibody at 1:540 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking ...read more
Immunocytochemistry/ Immunofluorescence: WNK3 Antibody [NBP2-93100] - Immunofluorescence analysis of U2OS cells using WNK3 Rabbit pAb (NBP2-93100) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: WNK3 Antibody [NBP2-93100] - Immunofluorescence analysis of NIH/3T3 cells using WNK3 Rabbit pAb (NBP2-93100) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: WNK3 Antibody [NBP2-93100] - Immunofluorescence analysis of PC-12 cells using WNK3 Rabbit pAb (NBP2-93100) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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WNK3 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 500-600 of human WNK3 (NP_065973.2). AGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WNK3
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for WNK3 Antibody - Azide and BSA Free

  • EC 2.7.11
  • FLJ30437
  • KIAA1566FLJ42662
  • PRKWNK3lysine deficient 3
  • WNK lysine deficient protein kinase 3

Background

Members of the 'with no lysine' (WNK) kinase family, such as WNK3, are serine-threonine protein kinases thatlack the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site.Instead, these kinases have a conserved lysine in subdomain I that is thought to provide this function (Holden et al.,2004 (PubMed 15194194)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional WNK3 Products

Array NBP2-93100

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Bioinformatics

Gene Symbol WNK3