![Western Blot: PHF8 Antibody [NB100-93313] - Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody NB100-93313 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PHF8-Antibody-Western-Blot-NB100-93313-img0009.jpg "Western Blot: PHF8 Antibody [NB100-93313] - Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody NB100-93313 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human PHD finger protein 8 (CAI41576.1). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PHF8 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ChIP-Seq 10 ug as per Ram O et al., Cell. 2011 Dec 23;147(7):1628-39. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PHF8-Antibody-Immunohistochemistry-Paraffin-NB100-93313-img0011.jpg "Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1 ug/ml). Detection: DAB")
![Western Blot: PHF8 Antibody [NB100-93313] - Detection of Human PHF8 on HeLa whole cell lysate using NB100-93313. PHF8 was also immunoprecipitated by rabbit anti-PHF8 antibody NB100-93314.](http://images.novusbio.com/fullsize/PHF8-Antibody-Western-Blot-NB100-93313-img0003.jpg "Western Blot: PHF8 Antibody [NB100-93313] - Detection of Human PHF8 on HeLa whole cell lysate using NB100-93313. PHF8 was also immunoprecipitated by rabbit anti-PHF8 antibody NB100-93314.")
![Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - Section of human small cell lung cancer. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PHF8-Antibody-Immunohistochemistry-Paraffin-NB100-93313-img0010.jpg "Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - Section of human small cell lung cancer. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1ug/ml). Detection: DAB")
![Western Blot WDR5 Antibody [Unconjugated]](https://images.novusbio.com/images2/WDR5_AF5810_Western_Blot_5854.jpg)
![Immunohistochemistry WDR5 Antibody [Unconjugated]](https://images.novusbio.com/images2/WDR5_AF5810_Immunohistochemistry_21711.jpg)
![Immunocytochemistry/ Immunofluorescence WDR5 Antibody [Unconjugated]](https://images.novusbio.com/images/af5810_human-mouse-wdr5-affinity-purified-polyclonal-ab-41202412401254.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")