![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Nuclear extract (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JMJD2A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Western-Blot-NB110-40585-img0007.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Nuclear extract (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JMJD2A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1010 and the C-terminus (residue 1064) of human Jumonji Domain Containing 2A using the numbering given in entry NP_055478.1 (GeneID 9682). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KDM4A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in ICC/IF reported in scientific literature (PMID 22373579), Use Use in ICC/IF reported in scientific literature ( 25645366). |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
Immunofluorescent staining | Human | 07/10/2018 |
Summary
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reviewed by:
Verified Customer |
WB | Human | 01/11/2012 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NB110-40585)Find related products by research area.
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| Verified Customer 07/10/2018 |
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| Application: | Immunofluorescent staining | |
| Species: | Human |
| Verified Customer 01/11/2012 |
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| Application: | WB | |
| Species: | Human |
| Gene Symbol | KDM4A |
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![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Lamins A/C-53BP1 interaction is dependent on the 53BP1 Tudor domain. U2OS/GFP-lamin A cells were subjected to IR (10 Gy) and allowed to recover for 1 h. Cell extracts were then subjected to immunoprecipitation using GFP-Trap beads, and bound complexes were then analyzed by immunoblotting using GFP, LAP2alpha and JMJD2A antibodies. WCE represents 1% input. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12258), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Western-Blot-NB110-40585-img0006.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Lamins A/C-53BP1 interaction is dependent on the 53BP1 Tudor domain. U2OS/GFP-lamin A cells were subjected to IR (10 Gy) and allowed to recover for 1 h. Cell extracts were then subjected to immunoprecipitation using GFP-Trap beads, and bound complexes were then analyzed by immunoblotting using GFP, LAP2alpha and JMJD2A antibodies. WCE represents 1% input. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12258), licensed under a CC-BY license.")
![Immunoprecipitation: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Detection of human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A by western blot of immunoprecipitates. Samples: Nuclear Extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40585 used for IP at 6 ug per reaction. Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A was also immunoprecipitated by rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40584. For blotting immunoprecipitated Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A, NB110-40585 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Immunoprecipitation-NB110-40585-img0005.jpg "Immunoprecipitation: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Detection of human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A by western blot of immunoprecipitates. Samples: Nuclear Extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40585 used for IP at 6 ug per reaction. Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A was also immunoprecipitated by rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40584. For blotting immunoprecipitated Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A, NB110-40585 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40585_rabbit-polyclonal-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-antibody-310202416235537.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40585_rabbit-polyclonal-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-antibody-310202416235544.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40585_rabbit-polyclonal-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-antibody-310202416235523.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
