Biological Strategies: Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell and animal models. HepG2 cells were incubated with a mixture of palmitic acid (PA) and ...read more
Biological Strategies: Western Blot: JMJD2B Antibody [NB100-74605] - Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Dilution used for WB at 0.5 ug/ml.
Independent Antibodies: Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B on HeLa whole cell lysate using NB100-74605. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody ...read more
Biological Strategies: Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified ...read more
Immunoprecipitation: JMJD2B Antibody [NB100-74605] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity ...read more
Genetic Strategies: Knockdown Validated: JMJD2B Antibody [NB100-74605] - Knockdown of JMJD2B reduces the intracellular TG level and represses PPARgamma2 expression in HepG2 cells. HepG2 cells in 6-well plates ...read more
Western Blot: JMJD2B Antibody [NB100-74605] - Gomisin N (GN) represses the expression of JMJD2B, PPAR gamma 2, & PPAR gamma 2 steatosis target genes in HepG2 cells. HepG2 cells were treated with GN (100 μM) for ...read more
Western Blot: JMJD2B Antibody [NB100-74605] - Overexpression of JMJD2B increases intracellular TG levels & stimulates PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were infected with Ad-GFP or ...read more
Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell & animal models. (A) HepG2 cells were incubated with a mixture of palmitic acid (PA) & oleic acid (OA) (1:2 ratio) at ...read more
Western Blot: JMJD2B Antibody [NB100-74605] - Knockdown of JMJD2B reduces the intracellular TG level & represses PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were transfected with scramble RNA or ...read more
Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell & animal models. (A) HepG2 cells were incubated with a mixture of palmitic acid (PA) & oleic acid (OA) (1:2 ratio) at ...read more
Western Blot: JMJD2B Antibody [NB100-74605] - Overexpression of JMJD2B increases intracellular TG levels & stimulates PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were infected with Ad-GFP or ...read more
Immunohistochemistry: JMJD2B Antibody [NB100-74605] - Adenovirus-mediated JMJD2B overexpression stimulated hepatic steatosis in vivo. C57BL/6 mice (8 weeks old) were injected with adenovirus Ad-GFP or Ad-JMJD2B. After ...read more
The immunogen recognized by this antibody maps to a region between residue 1056 and 1096 of human jumonji domain containing 2B using the numbering given in entry NP_055830.1 (GeneID 23030).
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM4B
Purity
Immunogen affinity purified
Innovator's Reward
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122 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3 using NB100-74605 in the following applications:
JmjC domain-containing histone demethylation protein 3B
JMJD2B
JMJD2BEC 1.14.11.-
jumonji domain containing 2B
Jumonji domain-containing protein 2B
KDM4B
KIAA0876
KIAA0876lysine-specific demethylase 4B
lysine (K)-specific demethylase 4B
Background
JMJD2B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. JMJD2B has been shown to be capable of antagonizing H3K9 trimethylation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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