![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0006.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse KCC2/SLC12A5. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC12A5 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 126 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for KCC2/SLC12A5 Antibody (NBP3-13161)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SLC12A5 |
![Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13161-img0003.jpg "Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Immunohistochemistry-Paraffin-NBP3-13161-img0002.jpg "Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0001.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500.")
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0004.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0005.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-91020242091487.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-9102024200103.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-91020242014613.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Human brain (5 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-910202420134099.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Human brain (5 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
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![Western Blot GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Western_Blot_19950.jpg)
![Simple Western GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Simple_Western_20168.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")