Vimentin Antibody

Vimentin is a type III intermediate filament (IF) protein, encoded by the VIM gene, that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cell such as endothelial cells, fibroblasts, and immune cells (1). Human vimentin monomer protein is 466 amino acids (aa) in length with a theoretical molecular weight of ~54 kDa (2). Vimentin protein contains a central rod domain composed of four alpha-helices which is flanked by an N-terminal head domain and C-terminal tail domain (1,3). Vimentin monomers dimerize to form more complex structures resulting in the assembly of eight tetramers, referred to as unit-length filaments, which then assemble into full-length intermediate filaments that are 10-12 nm wide (1,3,4).

Activated macrophages have been shown to secrete phosphorylated vimentin which can be stimulated by a variety of pathophysiological factors including oxidized low-density lipoproteins and TNF-alpha or inhibited by IL-10 (1). The vimentin protein is often expressed at the cell surface playing a role in cell-cell interactions, tissue damage and repair, immune response, and pathogen recognition (1). Vimentin functions in many cytoskeletal processes including cell migration, which is highlighted by its upregulation during epithelial-to-mesenchymal transition (EMT) (4,5). Vimentin is a commonly used marker for EMT and is expressed by many tumor types (5). For example, high metastasis of oral squamous cell carcinomas also showed high vimentin positive expression in immunohistochemical staining analysis (5). A number of vimentin targeting compounds are in cancer-related clinical trials, however, given the multifunctional role of vimentin, the effect of inhibition on non-malignant cells needs to be thoroughly examined (5).

References

1. Ramos, I., Stamatakis, K., Oeste, C. L., & Perez-Sala, D. (2020). Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21134675

2. Uniprot (P08670)

3. Morrow, C. S., & Moore, D. L. (2020). Vimentin's side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton (Hoboken, N.J.). https://doi.org/10.1002/cm.21645

4. van Bodegraven, E. J., & Etienne-Manneville, S. (2020). Intermediate filaments against actomyosin: the david and goliath of cell migration. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2020.05.006

5. Strouhalova, K., Prechova, M., Gandalovicova, A., Brabek, J., Gregor, M., & Rosel, D. (2020). Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers. https://doi.org/10.3390/cancers12010184

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Bovine, Guinea Pig.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NBP1-31327	Applications	Species
Fulong Yu, Liang Li, Yimei Gu, Song Wang, Lianbang Zhou, Xiaohu Cheng, Heng Jiang, Yang Huang, Yingfeng Zhang, Wenbao Qian, Xianghua Li, Zhining Liu Lysine demethylase 5C inhibits transcription of prefoldin subunit 5 to activate c-Myc signal transduction and colorectal cancer progression Molecular Medicine 2024-01-12 [PMID: 38216914]
Yu Z, Dong X, Song M et al. Targeting UBR5 inhibits postsurgical breast cancer lung metastases by inducing CDC73 and p53 mediated apoptosis International journal of cancer 2023-10-19 [PMID: 37855385]
Taggi M, Liuzzi F, Botticelli L et al. Evidence for the expression of vasorin in the human female reproductive tissues Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology 2023-06-09 [PMID: 37331376] (ICC/IF, Human) Details: 1:200 ICC/IF dilution	ICC/IF	Human
Huidrom LD, Dhanaji SN, Pandey S et al. Embryo-Uterine Cross-Talk: Exploration of the Immunomodulatory Mechanism in Buffalo Animals : an open access journal from MDPI 2022-11-14 [PMID: 36428366] (ICC/IF, Bovine)	ICC/IF	Bovine
Nagel A, Popeda M, Muchlinska A et al. ERalpha36-High Cancer-Associated Fibroblasts as an Unfavorable Factor in Triple-Negative Breast Cancer Cancers 2022-04-15 [PMID: 35454913]
She M, Li T, Shi W et al. AREG is involved in scleral remodeling in form-deprivation myopia via the ERK1/2-MMP-2 pathway FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-05-01 [PMID: 35436023] (ICC/IF, Guinea Pig)	ICC/IF	Guinea Pig
H LD, D SN, Pandey S et al. Impact of uterine epithelial cells and its conditioned medium on the in vitro embryo production in buffalo (Bubalusbubalis) Theriogenology 2022-02-18 [PMID: 35219005] (ICC/IF, Bovine)	ICC/IF	Bovine
Li Z, Zhou Z, Wu X et al. LMP1 promotes nasopharyngeal carcinoma metastasis through NTRK2-mediated anoikis resistance Am J Cancer Res 2020-07-01 [PMID: 32775002] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Zhou Z, Zhou Q, Wu X, et al. VCAM-1 Secreted from Cancer-Associated Fibroblasts Enhances the Growth and Invasion of Lung Cancer Cells through AKT and MAPK Signaling Cancer Letters 2020-01-01 [PMID: 31904479] (WB)	WB
Ren W, Hou J, Yang C et al. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res. 2019-02-08 [PMID: 30736829] (WB, Human)	WB	Human
Zuppinger C, Gibbons G et al. Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes. Eur J Histochem 2017-06-21 [PMID: 28735524] (ICC/IF, Human)	ICC/IF	Human
Inamdar SM, Hsu SC, Yeaman C. Probing Functional Changes in Exocyst Configuration with Monoclonal Antibodies. Front Cell Dev Biol 2016-06-03 [PMID: 27376061] (WB)	WB
Cho CK, Smith CR, Diamandis EP. Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery. J Proteome Res 2010-07-01 [PMID: 20459121] (WB, Human)	WB	Human
Show All 13 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Claudia Klein	ICC	Equine	12/11/2018	View Summary Application Immunocytochemistry Sample Tested fibroblasts Species Equine Lot ? Comments Comments I will have to look up the lot number and supply tomorrow. Staining material: cellular aggregates composed of equine endometrial fibroblast only. Co-staining using vimentin antibody (purchase order number: 3641031 from NovusBio website directly) and a cytokeratin antibody (mouse monoclonal) we have in the lab. Equine endometrial fibroblast express vimentin only. The image on the left shows the staining for vimentin and DAPI. Cytoplasmic staining is evident colored in red. Nuclei are stained with DAPI colored in blue. No staining for vimentin present. The images on the right hand site depict the negative control (i.e. omission of the primary antibody, DAPI only).
Enlarge	5 reviewed by: Christian Zuppinger	IF	Human	07/13/2016	View Summary Application Immunofluorescence Sample Tested primary human cells Species Human Lot 42095
	5 reviewed by: Verified Customer	WB	Human	09/02/2015	View Summary Application Western Blot Sample Tested Human cancer cell Species Human

Array NBP1-31327

• Vimentin Antibodies
• Vimentin ELISA Kits
• Vimentin Lysate
• Vimentin Proteins
• Vimentin Proteins and Enzyme