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VGLUT1 Antibody

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Western Blot: VGLUT1 Antibody [NB100-1837] - Cell lysate from rat

Product Details

Summary
Reactivity Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, Func, IHC, MS
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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VGLUT1 Antibody Summary

Immunogen
A 17 residue synthetic peptide derived from C-terminal domain of rat VGLUT1.
Localization
Cell Membrane
Specificity
Reacts with rat 55-60 kDa VGLUT1 protein of BON cells stably expressing VGLUT1 and of total rat brain extracts. No cross reactivity was detected with VGLUT2 and VGLUT3
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC17A7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy 1:200
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Radioimmunoassay 1:500
  • Western Blot 1:500
Publications
Read Publications using
NB100-1837 in the following applications:

Reactivity Notes

Not yet tested in other species. Use in Canine reported in scientific literature (PMID:18558516).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris 0,1M, glycine 0,1M, sucrose 2%.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Lyophilized powder, reconstituted in distilled water.

Alternate Names for VGLUT1 Antibody

  • BNPI
  • BNPIbrain-specific Na-dependent inorganic phosphate cotransporter
  • Brain-specific Na(+)-dependent inorganic phosphate cotransporter
  • member 7
  • SLC17A7
  • solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter)
  • Solute carrier family 17 member 7
  • VGLUT1
  • VGLUT1vesicular glutamate transporter 1

Background

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VGLUT1 Antibody (NB100-1837)(2)

We have publications tested in 2 confirmed species: Rat, Canine.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(2)
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Rat
(1)
Canine
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SLC17A7
Entrez
Uniprot