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VAP-A Antibody

Images

 
Genetic Strategies: Western Blot: VAP-A Antibody [NBP1-83132] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: VAP-A Antibody [NBP1-83132] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-83132] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: VAP-A Antibody [NBP1-83132] - Analysis in human cell line MOLT-4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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VAP-A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NDKLGITPPGNAPTVTSMSSINNTVATPASYHTKDDPRGLSVLKQEKQKNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASF
Localization
Endoplasmic Reticulum
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VAPA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
VAP-A Protein (NBP1-83132PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-83132 in the following applications:

Reactivity Notes

Rat (84%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VAP-A Antibody

  • hVAP-33
  • MGC3745,33 kDa VAMP-associated protein
  • VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
  • VAMP-A
  • VAP-33
  • VAP33VAMP (vesicle-associated membrane protein)-associated protein A (33kD)
  • VAPA
  • VAP-A
  • VAP-AVAMP-associated protein A
  • vesicle-associated membrane protein-associated protein A

Background

VAPA is encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-83132
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Applications: WB, ICC/IF, IHC, KD

Publications for VAP-A Antibody (NBP1-83132) (0)

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Review for VAP-A Antibody (NBP1-83132) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-83132:
Filter by Applications
IF
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunofluorescence VAP-A NBP1-83132
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5
reviewed by:
Verified Customer
IF Human 12/11/2017
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Summary

ApplicationImmunofluorescence
Sample Testedhela cell
SpeciesHuman
LotD114296

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VAP-A Antibody (NBP1-83132) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Verified Customer
12/11/2017
Application: IF
Species: Human

Bioinformatics

Gene Symbol VAPA
Entrez
Uniprot