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COL4A3BP Antibody

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Western Blot: COL4A3BP Antibody [NBP1-31118] - Sample (50 ug of whole cell lysate) A: Mouse white adipose 7. 5% SDS PAGE diluted at 1:1000.
Western Blot: COL4A3BP Antibody [NBP1-31118] - Sample (50 ug of whole cell lysate) A: Mouse white adipose 7. 5% SDS PAGE diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: COL4A3BP Antibody [NBP1-31118] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: COL4A3BP Antibody [NBP1-31118] - BT483 xenograft. COL4A3BP antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: COL4A3BP Antibody [NBP1-31118] - A: Hep G2 B: MOLT4 C: Raji 12% SDS PAGE; antibody diluted at 1:500

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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COL4A3BP Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COL4A3BP. The exact sequence is proprietary.
Localization
Cytoplasm, Golgi apparatus, Endoplasmic reticulum
Predicted Species
Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CERT1
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for COL4A3BP Antibody

  • Ceramide transfer protein
  • CERTFLJ20597
  • CERTL
  • collagen type IV alpha-3-binding protein
  • collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
  • Goodpasture antigen-binding protein
  • GPBPSTARD11ceramide transporter
  • hCERT
  • lipid-transfer protein CERTL
  • StARD11
  • StAR-related lipid transfer (START) domain containing 11
  • StAR-related lipid transfer protein 11
  • START domain containing 11
  • START domain-containing protein 11

Background

COL4A3BP is a kinase that specifically phosphorylates the N terminal region of the non collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Two transcripts exist for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Bioinformatics

Gene Symbol CERT1