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RTN3 Antibody

Images

 
Independent Antibodies: Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human cerebral cortex, colon, liver and lymph node using Anti-RTN3 antibody (A) NBP1-88871 shows similar protein ...read more
Immunocytochemistry/ Immunofluorescence: RTN3 Antibody [NBP1-88871] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human colon shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human lymph node shows no positivity in lymphoid cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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RTN3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IMTSSFLSSSEIHNTGLTILHGEKSHVLGSQPILAKEGKDHLDLLDMKKMEKPQGTSNNVSDSSVSLAAGVHCDRPSIPASFPEHPAFLSKKIGQVEEQIDKETKNPNGVSSREAKTALDADDRFTLLTAQKPPTEY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RTN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RTN3 Protein (NBP1-88871PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RTN3 Antibody

  • ASYIPASY interacting protein
  • HAP
  • isoforme III
  • isoforme VI
  • Neuroendocrine-specific protein-like 2
  • Neuroendocrine-specific protein-like II
  • NSPL2NSP-like protein II
  • NSPLIIhomolog of ASY protein
  • NSP-like protein 2
  • reticulon 3
  • reticulon-3
  • RTN3-A1

Background

May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis. There are 5 isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RTN3