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VAP-A Antibody (OTI10E10)

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Western Blot: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VAP-A.
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: VAP-A Antibody (10E10) [NBP2-46319] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI10E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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VAP-A Antibody (OTI10E10) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 2-227 of human VAPA (NP_919415) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VAPA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
27.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VAP-A Antibody (OTI10E10)

  • hVAP-33
  • MGC3745,33 kDa VAMP-associated protein
  • VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
  • VAMP-A
  • VAP-33
  • VAP33VAMP (vesicle-associated membrane protein)-associated protein A (33kD)
  • VAPA
  • VAP-A
  • VAP-AVAMP-associated protein A
  • vesicle-associated membrane protein-associated protein A

Background

VAPA is encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol VAPA