VAP-A Antibody (604101)

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Vesicle-associated membrane protein (VAMP)-associated protein A (VAP-A; also VAMP-A and VAP-33) is a 33 kDa, ubiquitously expressed, type IV transmembrane protein belonging to the VAP family of proteins (1). It is found in plasma and ER membranes as well as in intracellular vesicles as a homodimer and a heterodimer with VAP-B. Human VAP-A is synthesized as a 249 amino acid (aa) precursor that contains a 227 aa cytoplasmic domain and a 21 aa transmembrane region. The cytoplasmic domain contains a mobile sperm protein (MSP) domain (aa 13-131) and a coiled-coil region (aa 169-205). Human VAP-A is 97% aa identical to mouse and rat VAP-A. VAP-A and VAP-B recruit FFAT (two phenylalanines in an acidic tract)-motif-containing proteins to the cytosolic surface of ER membranes through a conserved region within their MSP domain, and they have been implicated in regulation of membrane transport, phospholipid biosynthesis, and the unfolded protein response (2, 3). Their ability to interact with lipid-transfer/binding proteins (LT/BPs) may affect the lipid composition of certain cellular membranes (2, 4). VAPs play a critical role in maintaining the structural and functional properties of the Golgi complex (2). Knockdown of VAP reduces the levels of phosphatidylinositol‑4‑phosphate (PI4P), diacylglycerol (DAG), and sphingomyelin (SM) in Golgi membranes and exports pleiotropic effects in Golgi-mediated transport (2). The effects of VAPs are mediated by their interacting FFAT-motif-containing proteins Nir2, OSBP, and CERT (2). VAPs provide a scaffold for these LT/BPs at the ER-Golgi membrane contact sites, thereby affecting the lipid composition of the Golgi membranes and consequently their structural and functional identities (2). VAP-A associates with and regulates the neurite outgrowth-promoting activity of protrudin, a protein that promotes neurite formation (5).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.

Showing Publications 1 - 3 of 3.

Publications using MAB5820	Applications	Species
D Höglinger, T Burgoyne, E Sanchez-He, P Hartwig, A Colaco, J Newton, CE Futter, S Spiegel, FM Platt, ER Eden NPC1 regulates ER contacts with endocytic organelles to mediate cholesterol egress Nat Commun, 2019-09-19;10(1):4276. 2019-09-19 [PMID: 31537798] (Western Blot, Human)	Western Blot	Human
D Mao, G Lin, B Tepe, Z Zuo, KL Tan, M Senturk, S Zhang, BR Arenkiel, M Sardiello, HJ Bellen VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway Autophagy, 2019-02-20;0(0):. 2019-02-20 [PMID: 30741620] (ICC, Human)	ICC	Human
Ben Johnson, Ashley N. Leek, Laura Solé, Emily E. Maverick, Tim P. Levine, Michael M. Tamkun Kv2 potassium channels form endoplasmic reticulum/plasma membrane junctions via interaction with VAPA and VAPB Proceedings of the National Academy of Sciences 2018-07-31 [PMID: 29941597]

Array MAB5820

• VAP-A Antibodies
• VAP-A Lysates
• VAP-A Proteins
• VAP-A Proteins and Enzyme