![Western Blot: VAP-A Antibody (4C12) [H00009218-M01] - VAPA monoclonal antibody (M01), clone 4C12. Analysis of VAPA expression in human placenta.](http://images.novusbio.com/fullsize/VAP-A-Antibody-4C12-Western-Blot-H00009218-M01-img0005.jpg "Western Blot: VAP-A Antibody (4C12) [H00009218-M01] - VAPA monoclonal antibody (M01), clone 4C12. Analysis of VAPA expression in human placenta.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 4C12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | VAPA (AAH02992, 1 a.a. ~ 242 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.MAKHEQILVLDPPTDLKFKGPFTDVVTTNLKLRNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGSTVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNTSDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASFRDNVTSPLPSLLVVIAAIFIGFFLGKFIL |
| Specificity | VAPA - VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa (4C12) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | VAPA |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00009218-M01 | Applications | Species |
|---|---|---|
| Barman B, Sung BH, Krystofiak E et al. VAP-A and its binding partner CERT drive biogenesis of RNA-containing extracellular vesicles at ER membrane contact sites Developmental cell 2022-04-25 [PMID: 35421371] |
Secondary Antibodies |
Isotype Controls |
Research Areas for VAP-A Antibody (H00009218-M01)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: VAP-A Antibody (4C12) [H00009218-M01] - Analysis of monoclonal antibody to VAPA on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/VAP-A-Antibody-4C12-Immunocytochemistry-Immunofluorescence-H00009218-M01-img0003.jpg "Immunocytochemistry/Immunofluorescence: VAP-A Antibody (4C12) [H00009218-M01] - Analysis of monoclonal antibody to VAPA on HeLa cell . Antibody concentration 10 ug/ml.")
![Western Blot: VAP-A Antibody (4C12) [H00009218-M01] - VAPA monoclonal antibody (M01), clone 4C12. Analysis of VAPA expression in K-562.](http://images.novusbio.com/fullsize/VAP-A-Antibody-4C12-Western-Blot-H00009218-M01-img0004.jpg "Western Blot: VAP-A Antibody (4C12) [H00009218-M01] - VAPA monoclonal antibody (M01), clone 4C12. Analysis of VAPA expression in K-562.")
![Western Blot: VAP-A Antibody (4C12) [H00009218-M01] - Analysis of VAPA expression in transfected 293T cell line by VAPA monoclonal antibody (M01), clone 4C12.Lane 1: VAPA transfected lysate(27.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/VAP-A-Antibody-4C12-Western-Blot-H00009218-M01-img0006.jpg "Western Blot: VAP-A Antibody (4C12) [H00009218-M01] - Analysis of VAPA expression in transfected 293T cell line by VAPA monoclonal antibody (M01), clone 4C12.Lane 1: VAPA transfected lysate(27.3 KDa).Lane 2: Non-transfected lysate.")
