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U2AF2 Antibody (SR1970)

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Western Blot: U2AF2 Antibody (SR1970) [NBP3-22034] - Western blot analysis using NBP3-22034 in Jurkat cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
SR1970
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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U2AF2 Antibody (SR1970) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human U2AF2 (Uniprot #: P26368)
Specificity
Detects endogenous levels of total U2AF2
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
U2AF2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for U2AF2 Antibody (SR1970)

  • hU2AF(65)
  • hU2AF65
  • splicing factor U2AF 65 kD subunit
  • splicing factor U2AF 65 kDa subunit
  • U2 (RNU2) small nuclear RNA auxiliary factor 2
  • U2 auxiliary factor 65 kDa subunit
  • U2 small nuclear ribonucleoprotein auxiliary factor (65kD)
  • U2 small nuclear RNA auxiliary factor 2
  • U2AF65U2 snRNP auxiliary factor large subunit

Background

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol U2AF2