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![Western Blot: U2AF2 Antibody [NBP2-33397] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/U2AF2-Antibody-Western-Blot-NBP2-33397-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: U2AF2 Antibody [NBP2-33397] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: U2AF2 Antibody [NBP2-33397] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/U2AF2-Antibody-Immunohistochemistry-NBP2-33397-img0001.jpg)
![Simple Western: U2AF2 Antibody [NBP2-33397] - Simple Western lane view shows a specific band for U2AF2 in 0.2 mg/ml of HEK 293T lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/U2AF2-Antibody-Simple-Western-NBP2-33397-img0005.jpg)
![Simple Western: U2AF2 Antibody [NBP2-33397] - Electropherogram image of the corresponding Simple Western lane view. U2AF2 antibody was used at 1:25 dilution on HEK 293T lysate(s) respectively.](http://images.novusbio.com/fullsize/U2AF2-Antibody-Simple-Western-NBP2-33397-img0006.jpg)
![Western Blot: U2AF2 Antibody [NBP2-20775] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/U2AF2-Antibody-Western-Blot-NBP2-20775-img0001.jpg "Western Blot: U2AF2 Antibody [NBP2-20775] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: U2AF2 Antibody [NBP2-20775] - Immunohistochemical analysis of paraffin-embedded OVCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/U2AF2-Antibody-Immunohistochemistry-Paraffin-NBP2-20775-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: U2AF2 Antibody [NBP2-20775] - U2AF2 antibody [C1C3] detects U2AF2 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: U2AF2 stained by U2AF2 antibody [C1C3] (NBP2-20775) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20775_rabbit-polyclonal-u2af2-antibody-91020241959355.jpg)
![Immunohistochemistry-Paraffin: U2AF2 Antibody [NBP2-20775] - U2AF2 antibody [C1C3] detects U2AF2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.U2AF2 stained by U2AF2 antibody [C1C3] (NBP2-20775) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20775_rabbit-polyclonal-u2af2-antibody-91020242075929.jpg)
![Immunohistochemistry-Paraffin: U2AF2 Antibody [NBP2-20775] - U2AF2 antibody [C1C3] detects U2AF2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.U2AF2 stained by U2AF2 antibody [C1C3] (NBP2-20775) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20775_rabbit-polyclonal-u2af2-antibody-91020242013408.jpg)
![Western Blot: U2AF2 Antibody (8E8R9) [NBP3-16432] - Western blot analysis of extracts of various cell lines, using U2AF2/U2AF2 Rabbit mAb (NBP3-16432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/U2AF2-Antibody-8E8R9-Western-Blot-NBP3-16432-img0003.jpg "Western Blot: U2AF2 Antibody (8E8R9) [NBP3-16432] - Western blot analysis of extracts of various cell lines, using U2AF2/U2AF2 Rabbit mAb (NBP3-16432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: U2AF2 Antibody (8E8R9) [NBP3-16432] - Immunohistochemistry of paraffin-embedded human colon using U2AF2/U2AF2 Rabbit mAb (NBP3-16432) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/U2AF2-Antibody-8E8R9-Immunohistochemistry-Paraffin-NBP3-16432-img0001.jpg)
![Western Blot: U2AF2 Antibody (8E8R9) [NBP3-16432] - Western blot analysis of extracts of various cell lines, using U2AF2/U2AF2 Rabbit mAb (NBP3-16432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/U2AF2-Antibody-8E8R9-Western-Blot-NBP3-16432-img0002.jpg)
![Immunoprecipitation: U2AF2 Antibody (8E8R9) [NBP3-16432] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug U2AF2 antibody (NBP3-16432). Western blot was performed from the immunoprecipitate using U2AF2 antibody (NBP3-16432) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/U2AF2-Antibody-8E8R9-Immunoprecipitation-NBP3-16432-img0004.jpg)
![Immunohistochemistry-Paraffin: U2AF2 Antibody [NBP2-04140] - Human breast carcinoma. Antibody: Affinity purified rabbit anti- U2AF65 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/U2AF2-Antibody-Immunohistochemistry-Paraffin-NBP2-04140-img0003.jpg "Immunohistochemistry-Paraffin: U2AF2 Antibody [NBP2-04140] - Human breast carcinoma. Antibody: Affinity purified rabbit anti- U2AF65 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: U2AF2 Antibody [NBP2-04140] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-U2AF65 antibody NBP2-04138 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. U2AF65 was also immunoprecipitated by rabbit anti-U2AF65 antibodies NBP2-04139 and NBP2-04140, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).](http://images.novusbio.com/fullsize/U2AF2-Antibody-Immunoprecipitation-NBP2-04140-img0002.jpg)
![Western Blot: U2AF2 Antibody (5G8) [H00011338-M03] - Analysis of U2AF2 expression in HepG2.](http://images.novusbio.com/fullsize/U2AF2-Antibody-5G8-Western-Blot-H00011338-M03-img0003.jpg "Western Blot: U2AF2 Antibody (5G8) [H00011338-M03] - Analysis of U2AF2 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: U2AF2 Antibody (5G8) [H00011338-M03] - Analysis of monoclonal antibody to U2AF2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/U2AF2-Antibody-5G8-Immunocytochemistry-Immunofluorescence-H00011338-M03-img0001.jpg)
![Sandwich ELISA: U2AF2 Antibody (5G8) [H00011338-M03] - Detection limit for recombinant GST tagged U2AF2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/U2AF2-Antibody-5G8-Sandwich-ELISA-H00011338-M03-img0002.jpg)
![Immunohistochemistry: U2AF2 Antibody [NBP2-04139] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- U2AF65 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/U2AF2-Antibody-Immunohistochemistry-NBP2-04139-img0002.jpg "Immunohistochemistry: U2AF2 Antibody [NBP2-04139] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- U2AF65 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: U2AF2 Antibody [NBP2-04139] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-U2AF65 antibody NBP2-04138 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. U2AF65 was also immunoprecipitated by rabbit anti-U2AF65 antibodies NBP2-04139 and NBP2-04140, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).](http://images.novusbio.com/fullsize/U2AF2-Antibody-Immunoprecipitation-NBP2-04139-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: U2AF2 Antibody [NBP2-04139] - Detection of human U2AF65 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-U2AF65 antibody NBP2-04139 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. U2AF65 was also immunoprecipitated by rabbit anti-U2AF65 antibodies NBP2-04139 and one other rabbit anti-U2AF65 antibody, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/U2AF2-Antibody-Immunoprecipitation-NBP2-04139-img0003.jpg)
![Western Blot: U2AF2 Antibody [NBP2-58989] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/U2AF2-Antibody-Western-Blot-NBP2-58989-img0002.jpg "Western Blot: U2AF2 Antibody [NBP2-58989] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: U2AF2 Antibody [NBP2-58989] - Staining of human cell line HEK 293 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/U2AF2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58989-img0001.jpg)
![Western Blot: U2AF2 Antibody (SR1970) [NBP3-22034] - Western blot analysis using NBP3-22034 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-22034_rabbit-u2af2-mab-sr1970-57202315545130.jpg "Western Blot: U2AF2 Antibody (SR1970) [NBP3-22034] - Western blot analysis using NBP3-22034 in Jurkat cell lysate.")
![Western Blot: U2AF2 Antibody (JE36-33) [NBP3-33071] - Western blot analysis of U2AF2 on different lysates with Rabbit anti-U2AF2 antibody (NBP3-33071) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: RAW264.7 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33071) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33071_rabbit-u2af2-mab-je36-33-288202414191521.jpg "Western Blot: U2AF2 Antibody (JE36-33) [NBP3-33071] - Western blot analysis of U2AF2 on different lysates with Rabbit anti-U2AF2 antibody (NBP3-33071) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: RAW264.7 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33071) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: U2AF2 Antibody (JE36-33) [NBP3-33071] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-U2AF2 antibody (NBP3-33071) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33071) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33071_rabbit-u2af2-mab-je36-33-298202414304133.jpg)
![Western Blot: U2AF2 Antibody [NBP1-57251] - Sample Tissue: Human Jurkat Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/U2AF2-Antibody-Western-Blot-NBP1-57251-img0004.jpg "Western Blot: U2AF2 Antibody [NBP1-57251] - Sample Tissue: Human Jurkat Antibody Dilution: 1.0 ug/ml")
![Western Blot: U2AF2 Antibody [NBP1-57251] - Reccomended Titration: 1.25 ug/ml Positive Control: Jurkat cell lysate U2AF2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells](http://images.novusbio.com/fullsize/U2AF2-Antibody-Western-Blot-NBP1-57251-img0005.jpg)
![Western Blot: U2AF2 Overexpression Lysate (Adult Normal) [NBP2-07511] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for U2AF2.](http://images.novusbio.com/fullsize/U2AF2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07511-img0001.jpg "Western Blot: U2AF2 Overexpression Lysate (Adult Normal) [NBP2-07511] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for U2AF2.")