![Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-2474] (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/TRIM5-Antibody-Immunohistochemistry-Paraffin-NB100-2474-img0002.jpg "Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-2474] (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence CNIEKNENYQPK corresponding to internal region according to NP_149023.1. |
| Specificity | This antibody is expected to recognise isoform alpha (NP_149023.1) only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TRIM5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Preliminary experiments gave an approx. 37 kDa band in Lysates of human cell lines Daudi, Jurkat and MOLT4 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3 kDa band according to NP_149023.1. The 37 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows cytoplasm staining in PCT. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot TRIM Antibody [Unconjugated]](https://images.novusbio.com/images2/TRIM_AF4708_Western_Blot_5528.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for TRIM5 Antibody (NB100-2474)Find related products by research area.
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| Gene Symbol | TRIM5 |
| Entrez |
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| Uniprot |
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