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TRIM13 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: TRIM13 Antibody [NBP1-85016] - Analysis in human testis and pancreas tissues. Corresponding TRIM13 RNA-seq data are presented for the same tissues.
Western Blot: TRIM13 Antibody [NBP1-85016] - Analysis in control (vector only transfected HEK293T lysate) and TRIM13 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: TRIM13 Antibody [NBP1-85016] - Staining of human testis shows moderate dot-like cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: TRIM13 Antibody [NBP1-85016] - Staining of human duodenum shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: TRIM13 Antibody [NBP1-85016] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: TRIM13 Antibody [NBP1-85016] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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TRIM13 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LSRLDTLETSKRKSLQLLTKDSDKVKEFFEKLQHTLDQKKNEILSDFETMKLAVMQAYDPEINKLNTILQEQRMAFNIAEAFKDVSEPIVFLQQMQEFREKIKVIKETPLPPSNLPASPLMKNFDTSQWEDIKLVDVDKLSLP
Predicted Species
Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
TRIM13 Protein (NBP1-85016PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TRIM13 Antibody

  • B-cell chronic lymphocytic leukemia tumor suppressor Leu5
  • CLL-associated RING finger
  • E3 ubiquitin-protein ligase TRIM13
  • EC 6.3.2.-
  • LEU5CAR
  • Leukemia-associated protein 5
  • Putative tumor suppressor RFP2
  • Ret finger protein 2RING finger protein 77
  • RFP2DLEU5
  • RNF77Leu5
  • tripartite motif containing 13
  • tripartite motif protein 13
  • tripartite motif-containing 13
  • Tripartite motif-containing protein 13

Background

Ret finger protein 2 This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This gene is located on chromosome 6. Multiple alternatively spliced transcript variants have been found for this gene. Mouse polyclonal antibody raised against a partial recombinant RFP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIM13