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ASAM Antibody

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Western blot shows lysates of mouse adipose (uterus) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASAM at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse adipose (uterus) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open ...read more
T98G human glioblastoma cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF7356-SP
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ASAM Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse ASAM
Thr18-Ala234
Accession # Q8R373
Specificity
Detects human and mouse recombinant  ASAM in direct ELISAs. Detects human and mouse ASAM in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CLMP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASAM Antibody

  • ACAM
  • ACAMCoxsackie- and adenovirus receptor-like membrane protein
  • Adipocyte adhesion molecule
  • adipocyte-specific adhesion molecule
  • ASAM
  • ASAMCAR-like membrane protein
  • Asp5
  • CLMP
  • CXADR-like membrane protein
  • FLJ22415

Background

ASAM (Adipocyte-specific adhesion molecule; also ACAM, CLMP, Asp5 and CXADR-like protein) is a 44-48 kDa member of the CTX family of molecules. It is expressed on adipocytes as well as select epithelial cells such as respiratory and intestinal epithelium, keratinocytes and Sertoli cells. ASAM appears to contribute to tight junction formation, and may promote the transition of preadipocytes to adipocytes. Mature mouse ASAM is a 356 amino acid (aa) type I transmembrane glycoprotein. It contains a 217 aa extracellular region (aa 18-234) that is characterized by the presence of two C2 type Ig-like domains (aa 18-126 and 134-223), and possesses a C-terminal 118 aa cytoplasmic domain. The extracellular region of mouse ASAM (aa 18-234) shares 97% and 100% aa sequence identity with human and rat ASAM, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CLMP
Uniprot