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Recombinant Human TNF RI/TNFRSF1A Protein

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SDS-Page: Recombinant Human TNF RI/TNFRSF1A Protein [NBP2-35274]
SDS-Page: Recombinant Human TNF RI/TNFRSF1A Protein [NBP2-35274]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35274-1mg
Carrier-Free
NBP2-35274-100ug
Carrier-Free
NBP2-35274-500ug
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Recombinant Human TNF RI/TNFRSF1A Protein Summary

Description
A single non-glycosylated polypeptide chain containing 190 amino acids corresponding to TNF RI/TNFRSF1A Source: E. coli

Uniprot ID: P19438

Amino Acid Sequence: IYPSGVIGLV PHLGDREKRD SVCPQGKYIH PQNNSICCTK CHKGTYLYND CPGPGQDTDC RECESGSFTA SENHLRHCLS CSKCRKEMGQ VEISSCTVDR DTVCGCRKNQ YRHYWSENLF QCFNCSLCLN GTVHLSCQEK QNTVCTCHAG FFLRENECVS CSNCKKSLEC TKLCLPQIEN VKGTEDSGTT

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Details of Functionality
TNF RI/TNFRSF1A protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 0.05 ug/ml, corresponding to a specific activity of > 2 x 10^4 IU/mg in the presence of 0.25 ng/mL of rHuTNF-alpha.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TNFRSF1A
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of TNF RI/TNFRSF1A as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
21.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human TNF RI/TNFRSF1A Protein

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • sTNF RI
  • sTNF RI/TNFRSF1A
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

TNF Receptor I (TNF RI), also named as TNFR-I, TNF R1, TNFRSF1A, p55 and p60, belongs to the tumor necrosis factor receptor superfamily, and it is encoded by the TNFRSF1A gene in human. TNF Receptor I (TNF RI)is a universally expressed protein and is thought to form homotrimers after ligand binding. TNF Receptor I (TNF RI) is one of the major receptors for the TNF-alpha. This receptor can activate the transcription factor NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Mature human soluble TNF RI shares 68 % ~ 73 % amino acid sequence identity with murine, rat, bovine and porcine soluble TNF RI.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TNFRSF1A
Uniprot