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TNF RI/TNFRSF1A Antibody (55R170) [Unconjugated]

 (18 Publications)
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Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB430). The ND50 is typically 5-20 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP, B/N
Clone
55R170
Clonality
Monoclonal
Host
Hamster
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB430-SP
Carrier Free
MAB430-500
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TNF RI/TNFRSF1A Antibody (55R170) [Unconjugated] Summary

Immunogen
Recombinant mouse TNF RI/TNFRSF1A
Extracellular domain
Specificity
Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human
(rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse (rm) CD27, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rmEDAR, rhFas, rmFas, rhEDAR, rhGITR, rmGITR, rhHVEM, rhLT beta R, rmLT beta R, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rhTNF RII or rmTNF RII is observed.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Hamster
Gene
TNFRSF1A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Neutralization 5-20 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB430 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF RI/TNFRSF1A Antibody (55R170) [Unconjugated]

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1‑3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6‑8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence, a 191 aa ECD with a PLAD domain (5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, porcine, human, and rat TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.

  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  4. Banner, D.W et al. (1993) Cell 73: 431.
  5. Chan, F.K. et al. (2000) Science 288:2351.
  6. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  7. Jin, L. et al. (2000) J. Immunol. 165:5153.
  8. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  9. Legler, D.F. et al. (2003) Immunity 18:655.
  10. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  11. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  12. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  13. McDermott, M.F. et al. (1999) Cell 97:133.
  14. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
  15. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TNF RI/TNFRSF1A Antibody (MAB430)(18)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Cell Culture, ICC, IHC, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Cell Culture
(1)
ICC
(1)
IHC
(2)
Immunoprecipitation
(1)
Neutralization
(5)
Western Blot
(4)
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Mouse
(12)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using MAB430 Applications Species
D Yu, T Li, JC Delpech, B Zhu, P Kishore, T Koshi, R Luo, KJB Pratt, G Popova, TJ Nowakowski, SA Villeda, X Piao Microglial GPR56 is the molecular target of maternal immune activation-induced parvalbumin-positive interneuron deficits Science Advances, 2022-05-06;8(18):eabm2545. 2022-05-06 [PMID: 35544642] (IHC, Mouse) IHC Mouse
Diankun Yu, Tao Li, Jean-Christophe Delpech, Beika Zhu, Priya Kishore, Tatsuhiro Koshi et al. Microglial GPR56 is the molecular target of maternal immune activation-induced parvalbumin-positive interneuron deficits Science Advances 5/11/2022 [PMID: 35522745]
Xinyue Huang, Shuixia Tan, Yanxia Li, Shuangyi Cao, Xingyan Li, Heling Pan et al. Caspase inhibition prolongs inflammation by promoting a signaling complex with activated RIPK1 Journal of Cell Biology 6/7/2021 [PMID: 33914027]
D Moujalled, P Gangatirka, M Kauppi, J Corbin, M Lebois, JM Murphy, N Lalaoui, JM Hildebrand, J Silke, WS Alexander, EC Josefsson The necroptotic cell death pathway operates in megakaryocytes, but not in platelet synthesis Cell Death & Disease, 2021-01-28;12(1):133. 2021-01-28 [PMID: 33510145] (ICC, IHC, Western Blot, Mouse) ICC, IHC, Western Blot Mouse
G Ebert, S Lopaticki, MT O'Neill, RWJ Steel, M Doerflinge, P Rajasekara, ASP Yang, S Erickson, L Ioannidis, P Arandjelov, L Mackiewicz, C Allison, J Silke, M Pellegrini, JA Boddey Targeting the Extrinsic Pathway of Hepatocyte Apoptosis Promotes Clearance of Plasmodium Liver Infection Cell Rep, 2020-03-31;30(13):4343-4354.e4. 2020-03-31 [PMID: 32234472] (Western Blot, Mouse) Western Blot Mouse
Lama AlAbdi, Debapriya Saha, Ming He, Mohd Saleem Dar, Sagar M. Utturkar, Putu Ayu Sudyanti et al. Oct4-Mediated Inhibition of Lsd1 Activity Promotes the Active and Primed State of Pluripotency Enhancers Cell Reports 2/4/2020 [PMID: 32023463]
L Shi, J Wang, N Ding, Y Zhang, Y Zhu, S Dong, X Wang, C Peng, C Zhou, L Zhou, X Li, H Shi, W Wu, X Long, C Wu, W Liao Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy Nat Commun, 2019-11-28;10(1):5421. 2019-11-28 [PMID: 31780645] (Cell Culture, Mouse) Cell Culture Mouse
Cerebral ischemia-induced angiogenesis is dependent on tumor necrosis factor receptor 1-mediated upregulation of ?5?1 and ?V?3 integrins J Neuroinflammation, 2016-09-01;13(1):227. 2016-09-01 [PMID: 27586239] (Neutralization, Mouse) Neutralization Mouse
Wertz I, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman R, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother W, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee W, Goodnow C, Balazs M, Yu K, Kolumam G, Dixit V Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation. Nature, 2015-12-09;528(7582):370-5. 2015-12-09 [PMID: 26649818] (Western Blot, Mouse) Western Blot Mouse
Goto Y, Ogawa K, Nakamura T, Hattori A, Tsujimoto M TLR-mediated secretion of endoplasmic reticulum aminopeptidase 1 from macrophages. J Immunol, 2014-03-31;192(9):4443-52. 2014-03-31 [PMID: 24688025] (Neutralization, Mouse) Neutralization Mouse
Show All 18 Publications.

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Bioinformatics

Gene Symbol TNFRSF1A