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TIF1 alpha Antibody (S01-4B4)

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Western Blot: TIF1 alpha Antibody (S01-4B4) [NBP3-19746] - Western blot detection of TIF1 alpha in Hela, A549 cell lysates using NBP3-19746 (1:1000 diluted). Predicted band size: 117kDa. Observed band size: 117kDa.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
S01-4B4
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

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TIF1 alpha Antibody (S01-4B4) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein of human TIF1 alpha (Uniprot # O15164)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TRIM24
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1/1000
Theoretical MW
117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for TIF1 alpha Antibody (S01-4B4)

  • E3 ubiquitin-protein ligase TRIM24
  • EC 6.3.2
  • EC 6.3.2.-
  • hTIF1
  • PTC6
  • RING finger protein 82
  • RNF82
  • RNF82Tif1a
  • TIF1 alpha
  • TIF1
  • TIF1A
  • TIF1-alpha
  • TIF1ATIF1ALPHA
  • TIF1TF1A
  • transcription intermediary factor 1-alpha
  • transcriptional intermediary factor 1
  • TRIM24
  • tripartite motif containing 24
  • tripartite motif-containing 24
  • Tripartite motif-containing protein 24

Background

TIF1 alpha is encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIM24