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![Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - TRIM24 monoclonal antibody (M01), clone 2F2. Analysis of TRIM24 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Western-Blot-H00008805-M01-img0011.jpg "Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - TRIM24 monoclonal antibody (M01), clone 2F2. Analysis of TRIM24 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of monoclonal antibody to TRIM24 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Immunocytochemistry-Immunofluorescence-H00008805-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of monoclonal antibody to TRIM24 on formalin-fixed paraffin-embedded human testis. Antibody concentration 6 ug/ml.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Immunohistochemistry-Paraffin-H00008805-M01-img0008.jpg)
![Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - TRIM24 monoclonal antibody (M01), clone 2F2 Analysis of TRIM24 expression in IMR-32.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Western-Blot-H00008805-M01-img0010.jpg)
![Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of TRIM24 expression in transfected 293T cell line by TRIM24 monoclonal antibody (M01), clone 2F2.Lane 1: TRIM24 transfected lysate(116.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Western-Blot-H00008805-M01-img0012.jpg)
![Immunoprecipitation: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of TRIM24 transfected lysate using anti-TRIM24 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TRIM24 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Immunoprecipitation-H00008805-M01-img0009.jpg)
![Western Blot: TIF1 alpha Antibody [NBP1-92506] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP1-92506-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TIF1 alpha Antibody [NBP1-92506] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody [NBP1-92506] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92506-img0006.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-92506-img0012.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts while additional moderate cytoplasmic in Leydig cells.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-92506-img0007.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human pancreas shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-92506-img0009.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP1-92506-img0010.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human placenta shows moderate nuclear and weak cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-92506-img0011.jpg)
![Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 Alpha/TRIM24 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TIF1 Alpha/TRIM24 antibody NB100-2596 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NB100-2596-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 Alpha/TRIM24 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TIF1 Alpha/TRIM24 antibody NB100-2596 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-TIF1alpha/TRIM24 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-2596-img0013.jpg)
![Chromatin Immunoprecipitation Sequencing: TIF1 alpha Antibody [NB100-2596] - Localization of TIF1 Alpha/TRIM24 Binding Sites by ChIP-sequencing.Chromatin from Breast cancer cell line T47D was immunoprecipitated with anti-TIF1 Alpha/TRIM24 antibody NB100-2596 and analyzed by DNA sequencing. The figure illustrates the peak distribution of TIF1 Alpha/TRIM24 binding within a 250 Kb region of chromosome 19 as detected using anti-TIF1 Alpha/TRIM24 antibody NB100-2596. ChIP-seq validation performed by Active Motif, Carlsbad, CA.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB100-2596-img0014.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 alpha/TRIM24 on HeLa whole cell lysate using NB100-2596. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2597.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NB100-2596-img0003.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-2596-img0012.jpg)
![Western Blot: TIF1 alpha Antibody [NB100-2597] - Detection of Human TIF1 alpha/TRIM24 on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lysate (50 microgram) using NB100-2597. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2596.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NB100-2597-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TIF1 alpha Antibody [NB100-2597] - Detection of Human TIF1 alpha/TRIM24 on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lysate (50 microgram) using NB100-2597. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2596.")
![Western Blot: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIF1.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Western-Blot-NBP2-46213-img0006.jpg "Western Blot: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIF1.")
![Immunohistochemistry: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Immunohistochemistry-NBP2-46213-img0005.jpg)
![Immunohistochemistry: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Immunohistochemistry-NBP2-46213-img0001.jpg)
![Immunohistochemistry: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Immunohistochemistry-NBP2-46213-img0002.jpg)
![Immunohistochemistry: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Immunohistochemistry-NBP2-46213-img0003.jpg)
![Immunohistochemistry: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Immunohistochemistry-NBP2-46213-img0004.jpg)
![Western Blot: TIF1 alpha Antibody [NBP2-41220] - Analysis of TRIM24 in A431 cell lysate with TRIM24 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP2-41220-img0004.jpg "Western Blot: TIF1 alpha Antibody [NBP2-41220] - Analysis of TRIM24 in A431 cell lysate with TRIM24 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody [NBP2-41220] - TIF1 in EL4 cells with TIF1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunofluorescence-NBP2-41220-img0003.jpg)
![Western Blot: TIF1 alpha Antibody [NBP2-41220] - Western blot analysis of TRIM24 in EL4 cell lysate with TRIM24 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP2-41220-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody [NBP2-41220] - Immunocytochemistry of TRIM24 in EL4 cells with TRIM24 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41220-img0002.jpg)
![Western Blot: TIF1 alpha Antibody [NB300-847] - Analysis of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NB300-847-img0004.jpg "Western Blot: TIF1 alpha Antibody [NB300-847] - Analysis of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB300-847] - (4ug/ml) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NB300-847-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Immunofluorescent Analysis of PFA-fixed MCF7 cells. TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by IgG-CF488 (green), counterstained with phalloidin.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-PCRP-TRIM24-1B12-Immunocytochemistry-Immunofluorescence-NBP3-07464-img0003.jpg "Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Immunofluorescent Analysis of PFA-fixed MCF7 cells. TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by IgG-CF488 (green), counterstained with phalloidin.")
![Flow Cytometry: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Flow Cytometric Analysis of PFA-fixed HeLa cells. TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-PCRP-TRIM24-1B12-Flow-Cytometry-NBP3-07464-img0002.jpg)
![Protein Array: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12).](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-PCRP-TRIM24-1B12-Protein-Array-NBP3-07464-img0001.jpg)
![Western Blot: TIF1 alpha Antibody [NBP2-56418] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP2-56418-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TIF1 alpha Antibody [NBP2-56418] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody [NBP2-56418] - Staining of human cell line Hep G2 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56418-img0001.jpg)
![Western Blot: TIF1 alpha Antibody (JE64-31) [NBP3-33052] - Western blot analysis of TIF1 alpha on different lysates with Rabbit anti-TIF1 alpha antibody at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 117 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody TRIM24 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33052_rabbit-tif1-alpha-mab-je64-31-288202412493491.jpg "Western Blot: TIF1 alpha Antibody (JE64-31) [NBP3-33052] - Western blot analysis of TIF1 alpha on different lysates with Rabbit anti-TIF1 alpha antibody at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 117 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody TRIM24 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TIF1 alpha Antibody (JE64-31) [NBP3-33052] - Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-TIF1 alpha antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody TRIM24 at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33052_rabbit-tif1-alpha-mab-je64-31-29820241411546.jpg)
![Western Blot: TIF1 alpha Antibody (S09-3G6) [NBP3-19977] - Western blot detection of TIF1 alpha in K562, Hela cell lysates using NBP3-19977 (1:1000 diluted). Predicted band size: 117kDa. Observed band size: 117kDa.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-S09-3G6-Western-Blot-NBP3-19977-img0001.jpg "Western Blot: TIF1 alpha Antibody (S09-3G6) [NBP3-19977] - Western blot detection of TIF1 alpha in K562, Hela cell lysates using NBP3-19977 (1:1000 diluted). Predicted band size: 117kDa. Observed band size: 117kDa.")
![Western Blot: TIF1 alpha Antibody (S01-4B4) [NBP3-19746] - Western blot detection of TIF1 alpha in Hela, A549 cell lysates using NBP3-19746 (1:1000 diluted). Predicted band size: 117kDa. Observed band size: 117kDa.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-S01-4B4-Western-Blot-NBP3-19746-img0001.jpg "Western Blot: TIF1 alpha Antibody (S01-4B4) [NBP3-19746] - Western blot detection of TIF1 alpha in Hela, A549 cell lysates using NBP3-19746 (1:1000 diluted). Predicted band size: 117kDa. Observed band size: 117kDa.")