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TdT Recombinant Protein Antigen

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

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TdT Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human TdT

Source: E.coli

Amino Acid Sequence: DEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSSWQEGKTWKAIRVDLVLCPYE

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
DNTT
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10-100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP3-21261. It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for TdT Recombinant Protein Antigen

  • deoxynucleotidyltransferase, terminal
  • DNTT
  • EC 2.7.7
  • EC 2.7.7.31
  • nucleosidetriphosphate:DNA deoxynucleotidylexotransferase
  • TdT
  • TDTDNA nucleotidylexotransferase
  • Terminal addition enzyme
  • Terminal deoxynucleotidyltransferase
  • terminal deoxyribonucleotidyltransferase
  • Terminal transferase

Background

TdT is a nuclear enzyme involved in the recombination of Immunoglobulin and T cell receptor genes. TdT is expressed in early lymphoid cells and by tumor cells in certain classes of acute lymphoid leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Additional TdT Products

Array NBP3-21261PEP

Research Areas for TdT Recombinant Protein Antigen (NBP3-21261PEP)

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Blogs on TdT.

TdT (Terminal Deoxynucleotidyl Transferase)
The enzyme family of DNA polymerases plays a fundamental role in the replication, repair, and recombination of nucleic acid. Its members include DNA Polymerase b (Pol b), DNA Polymerase g (Pol g), and DNA Polymerase m (Pol m). TdT is a very unique and...  Read full blog post.

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Bioinformatics

Gene Symbol DNTT