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SUMO1 Antibody - BSA Free

Images

 
Western Blot: SUMO1 Antibody [NBP1-76737] - HL-60 cell lysate with sumo antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody [NBP1-76737] - Sumo in HL60 cells with Sumo antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody [NBP1-76737] - HL60 cells with Sumo 1 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ChSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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SUMO1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human sumo. The immunogen is located within the first 50 amino acids of Sumo. Amino Acid Squence: EAKPSTEDLGDKKE
Predicted Species
Porcine (100%), Bovine (100%), Chicken (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SUMO1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-2 ug/ml
Control Peptide
SUMO1 Peptide (NBP1-76737PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for SUMO1 Antibody - BSA Free

  • DAP1
  • GAP modifying protein 1
  • GAP-modifying protein 1
  • GMP1SMT3CSMT3H3OFC10UBL1PIC1
  • PIC1
  • SENP2
  • Sentrin
  • small ubiquitin-related modifier 1
  • SMT3 homolog 3
  • SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
  • SMT3 suppressor of mif two 3 homolog 1 (yeast)
  • SMT3
  • SMT3C
  • SMT3H3
  • SUMO1
  • SUMO-1
  • Ubiquitin-homology domain protein PIC1
  • ubiquitin-like 1 (sentrin)
  • Ubiquitin-like protein SMT3C
  • Ubiquitin-like protein UBL1
  • UBL1

Background

The sumo family of proteins is related both structurally and functionally to ubiquitin in that they are post-translationally attached to the e-amino group of a lysine residue of the substrate protein. This sumoylation plays a number of roles in DNA replication and repair, protein targeting to various subnuclear structures, and the regulation of numerous cellular processes including the inflammatory response in mammalian cells. Sumo was initially identified as a covalent modification of RanGAP1 in studies on nuclear import in mammalian cells. More recently, sumo has been shown to be involved in the regulation of transcription factors, possibly by enhancing their interactions with co-repressors. Sumo is also thought to play some role in the modulation of ubiquitin-mediated degradation of proteins by acting as an inhibitor. At least four different isoforms of sumo are known to exist; Sumo antibody will only recognize isoform 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SUMO1
Uniprot