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STEAP3/TSAP6 Antibody

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Orthogonal Strategies: Western Blot: STEAP3/TSAP6 Antibody [NBP2-13395] - Analysis in human cell line PC-3 and human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: STEAP3/TSAP6 Antibody [NBP2-13395] - Staining of human cell line U-2 OS shows localization to nucleoli & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: STEAP3/TSAP6 Antibody [NBP2-13395] - Staining of human fallopian tube shows moderate membranous and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: STEAP3/TSAP6 Antibody [NBP2-13395] - Staining of human liver shows strong cytoplasmic and membranous positivity in hepatocytes.
Immunohistochemistry-Paraffin: STEAP3/TSAP6 Antibody [NBP2-13395] - Staining of human breast shows strong membranous and cytoplasmic positivity in glnadular cells.
Immunohistochemistry-Paraffin: STEAP3/TSAP6 Antibody [NBP2-13395] - Staining of human duodenum shows moderate cytoplasmic and membranous positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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STEAP3/TSAP6 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: PKRTARLFPSAAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLSDQLAGKILVDVSNPTEQEHLQHRE
Predicted Species
Mouse (93%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STEAP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
STEAP3/TSAP6 Protein (NBP2-13395PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for STEAP3/TSAP6 Antibody

  • AHMIO2
  • dudlin 2
  • Dudlin-2
  • dudulin 2
  • dudulin-2
  • Dudulin-2,1010001D01Rik
  • EC 1.16.1.-
  • hpHyde
  • hTSAP6
  • metalloreductase STEAP3
  • pHyde
  • six transmembrane prostate protein 3
  • Six-transmembrane epithelial antigen of prostate 3
  • STEAP family member 3
  • STEAP3
  • STMP3
  • TSAP6
  • TSAP6STMP3
  • tumor suppressor pHyde
  • Tumor suppressor-activated pathway protein 6

Background

STEAP3 is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell cell junctions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STEAP3