STEAP1 Antibody - BSA Free Summary
Immunogen |
Antibody was raised against a 16 amino acid synthetic peptide from near the center of human STEAP1. The immunogen is located within amino acids 130 - 180 of STEAP1. Amino Acid Squence: LHNGTKYKKFPHWLDK |
Specificity |
This STEAP1 antibody does not cross-react with other STEAP proteins. |
Predicted Species |
Porcine (94%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
STEAP1 |
Purity |
Peptide affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:100-1:2000
- Immunocytochemistry/ Immunofluorescence 20 ug/ml
- Immunohistochemistry 2.5 ug/ml
- Immunohistochemistry-Paraffin 2.5 ug/ml
- Western Blot 1-2 ug/ml
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Control Peptide |
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Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.02% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Peptide affinity purified |
Alternate Names for STEAP1 Antibody - BSA Free
Background
The six-transmembrane epithelial antigen of prostate 1 (STEAP1) was the first member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. The normal function of STEAP is still uncertain; unlike other members of the STEAP family, STEAP1 does not promote iron or copper reduction or uptake and lacks the FNO-like reductase domain critical for activity. However, its expression is highly increased in multiple cancer cell lines, including prostate, bladder, colon, and ovarian cancers. Supporting this is evidence that STEAP1 peptides can be used to stimulate CD8+ T cells from healthy donors, enabling them to recognize STEAP1-positive human tumor cells, suggesting that STEAP1 may a potential target for cancer immunotherapy. At least three isoforms of STEAP1 are known to exist. This STEAP1 antibody does not cross-react with other STEAP proteins.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for STEAP1 Antibody (NBP1-76822). (Showing 1 - 1 of 1 FAQ).
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I’m interessted in your STEP1 antibody (NBP1-76822). Could you precise what kind of affinity purified step did you perform to get the antibody ? would you have any further details on ELISA assays ?
- This antibody was purified by using affinity chromatography using the peptide immunogen. What information are you looking for in ELISA? We recommend a starting dilution of 1:100-1:2000.
Secondary Antibodies
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Isotype Controls
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