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STAMP2/STEAP4 Antibody

 (4 Publications)
 (1 Review)
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Western Blot: STAMP2/STEAP4 Antibody [NB100-68162] - HEK293 overexpressing Dudulin4 and probed with (mock transfection in first lane).
Western Blot: STAMP2/STEAP4 Antibody [NB100-68162] - HEK293 overexpressing Dudulin4 and probed with (mock transfection in first lane).
Western Blot: STAMP2/STEAP4 Antibody [NB100-68162] - (0.3ug/ml) Analysis of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunoprecipitation: STAMP2/STEAP4 Antibody [NB100-68162] - Analysis of STEAP4 in MES13 cells using anti-STEAP4 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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STAMP2/STEAP4 Antibody Summary

Immunogen
Peptide with sequence CVDNTLTRIRQGWERN corresponding to C-Terminus according to NP_078912.2.
Predicted Species
Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STEAP4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:10-1:500
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 50-55 kDa band observed in human adipose lysates (calculated MW of 52.0 kDa band according to NP_078912.2). In transfected HEK293 transiently expressing human Dudulin4 a band of approx. 52 kDa is observed. This band is not observed in the non-transfected HEK293. IP reactivity per customer review.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
STAMP2/STEAP4 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-68162 in the following applications:
Publications
Read Publications using
NB100-68162 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in (PMID:29078383).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STAMP2/STEAP4 Antibody

  • EC 1.16.1
  • EC 1.16.1.-
  • FLJ23153
  • Six-transmembrane epithelial antigen of prostate 4
  • SixTransMembrane protein of prostate 2
  • STAMP2
  • STAMP2DKFZp666D049
  • STEAP family member 4
  • STEAP4
  • TIARP
  • TIARPsix transmembrane prostate protein 2
  • TNFAIP9
  • Tumor necrosis factor, alpha-induced protein 9metalloreductase STEAP4
  • tumor necrosis-alpha-induced adipose-related protein

Background

STEAP4 is a member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. It is similar to two other members of the STEAP family, STEAP2 and STEAP3. STEAP4 promotes the reduction of both iron (Fe3+ to Fe2+) and copper (Cu2+ to Cu1+). STEAP4 is highly expressed in placenta, lung, heart and prostate tissues. Furthermore, it is over expressed in prostate cancer cells compared to normal prostate cells. STEAP4 may have a role in cell proliferation and prostrate cancer progression since over expression of STEAP4 in prostate cells significantly increases cell growth and colony formation. At least two isoforms of this protein are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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STAMP2/STEAP4
Antibody
NB100-68162
STAMP2/STEAP4 Antibody
Species: Hu, Mu, Rt, Ca
Applications: WB, ELISA, IP

Publications for STAMP2/STEAP4 Antibody (NB100-68162)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for STAMP2/STEAP4 Antibody (NB100-68162) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-68162:
Filter by Applications
IP
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunoprecipitation STAMP2/STEAP4 NB100-68162
Enlarge 		5
reviewed by:
Verified Customer
 IP Mouse 05/11/2015
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Summary

ApplicationImmunoprecipitation
Sample TestedMES13 cells
SpeciesMouse

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Video Protocols

WB Video Protocol

FAQs for STAMP2/STEAP4 Antibody (NB100-68162) (0)

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Control Lysate(s)

STAMP2/STEAP4 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Immunoprecipitation STAMP2/STEAP4 NB100-68162
Enlarge
 Verified Customer
05/11/2015
Application: IP
Species: Mouse
read this review

Bioinformatics

Gene Symbol STEAP4
Entrez
Uniprot