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PKMYT1 Antibody (OTI5E1)

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Western Blot: PKMYT1 Antibody (5E1) [NBP2-02275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.
Western Blot: PKMYT1 Antibody (5E1) [NBP2-02275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: PKMYT1 Antibody (OTI5E1) [NBP2-02275] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKMYT1.
Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human thyroid tissue using anti-PKMYT1 mouse monoclonal antibody.
Flow Cytometry: PKMYT1 Antibody (5E1) [NBP2-02275] - Analysis of Jurkat cells, using anti-PKMYT1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PKMYT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PKMYT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PKMYT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human breast tissue using anti-PKMYT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human endometrium tissue using anti-PKMYT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human Kidney tissue using anti-PKMYT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human liver tissue using anti-PKMYT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human prostate tissue using anti-PKMYT1 mouse monoclonal antibody.
Flow Cytometry: PKMYT1 Antibody (5E1) [NBP2-02275] - Analysis of Hela cells, using anti-PKMYT1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI5E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73452AF350
Alexa Fluor 405
NBP2-73452AF405
Alexa Fluor 488
NBP2-73452AF488
Alexa Fluor 532
NBP2-73452AF532
Alexa Fluor 594
NBP2-73452AF594
Alexa Fluor 647
NBP2-73452AF647
Alexa Fluor 700
NBP2-73452AF700
Alexa Fluor 750
NBP2-73452AF750
Allophycocyanin
NBP2-73452APC
Allophycocyanin/Cy7
NBP2-73452APCCY7
Biotin
NBP2-73452B
CoraFluor 1
NBP2-73452CL1
DyLight 350
NBP2-73452UV
DyLight 405
NBP2-73452V
DyLight 488
NBP2-73452G
DyLight 550
NBP2-73452R
DyLight 594
NBP2-73452DL594
DyLight 650
NBP2-73452C
DyLight 680
NBP2-73452FR
DyLight 755
NBP2-73452IR
FITC
NBP2-73452F
HRP
NBP2-73452H
Janelia Fluor 525
NBP2-73452JF525
Janelia Fluor 549
NBP2-73452JF549
Janelia Fluor 646
NBP2-73452JF646
Janelia Fluor 669
NBP2-73452JF669
PE
NBP2-73452PE
PE/Atto594
NBP2-73452PEATT594
PE/Cy5.5
NBP2-73452PECY55
PE/Cy7
NBP2-73452PECY7
PerCP
NBP2-73452PCP
mFluor Violet 450 SE
NBP2-73452MFV450
mFluor Violet 500 SE
NBP2-73452MFV500
mFluor Violet 610 SE
NBP2-73452MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-73452
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PKMYT1 Antibody (OTI5E1) Summary

Immunogen
Full length human recombinant protein of human PKMYT1 (NP_004194) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PKMYT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
Theoretical MW
54.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKMYT1 Antibody (OTI5E1)

  • DKFZp547K1610
  • EC 2.7.11.1
  • FLJ20093
  • Myt1 kinase
  • MYT1membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
  • protein kinase, membrane associated tyrosine/threonine 1

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PKMYT1 Antibody
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NBP2-02275
PKMYT1 Antibody (OTI5E1)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC

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Bioinformatics

Gene Symbol PKMYT1
Entrez