Home » STAT5a/b » STAT5a/b Primary Antibodies » STAT5a/b [p Tyr694, p Tyr699] Antibody (1247C) [Unconjugated]

STAT5a/b [p Tyr694, p Tyr699] Antibody (1247C) [Unconjugated]

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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB41901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT5a/b at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for ...read more
Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Rabbit anti-Human Phospho-STAT5a/b (Y694/Y699) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
1247C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC41901U-100UG
Alexa Fluor 405
IC41901V-100UG
Alexa Fluor 488
IC41901G-100UG
Alexa Fluor 532
FAB41901X
Alexa Fluor 594
IC41901T-100UG
Alexa Fluor 647
IC41901R-100UG
Alexa Fluor 700
IC41901N-100UG
Alexa Fluor 750
IC41901S-100UG
Allophycocyanin
FAB41901A
Biotin
FAB41901B
CoraFluor 1
FAB41901CL1
DyLight 350
FAB41901D
DyLight 405
FAB41901E
DyLight 488
FAB41901K
DyLight 550
FAB41901L
DyLight 594
FAB41901M
DyLight 650
FAB41901W
DyLight 680
FAB41901Y
DyLight 755
FAB41901Z
FITC
FAB41901F
HRP
FAB41901H
Janelia Fluor 525
FAB41901JF525
Janelia Fluor 549
FAB41901I
Janelia Fluor 646
FAB41901J
Janelia Fluor 669
FAB41901JF669
PE
FAB41901P
PerCP
FAB41901C
mFluor Violet 450 SE
FAB41901MFV450
mFluor Violet 500 SE
FAB41901MFV500
mFluor Violet 610 SE
FAB41901MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB41901-SP
Carrier Free
IC41901V-100UG
Carrier Free
IC41901U-100UG
Carrier Free
IC41901T-100UG
Carrier Free
IC41901S-100UG
Carrier Free
IC41901R-100UG
Carrier Free
IC41901N-100UG
Carrier Free
IC41901G-100UG
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STAT5a/b [p Tyr694, p Tyr699] Antibody (1247C) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Phosphopeptide containing human STAT5b Y699 site (amino acid sequence of this peptide is identical to a corresponding region of human STAT5a containing Y694)
Accession # P51692
Modification
p Tyr694, p Tyr699
Specificity
Detects human STAT5a/b when phosphorylated at Y964/Y699 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publication using MAB41901.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT5a/b [p Tyr694, p Tyr699] Antibody (1247C) [Unconjugated]

  • MGF
  • STAT5
  • STAT5A signal transducer and activator of transcription 5A
  • STAT5a/b

Background

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL‑22, IL-6, IFN-alpha ). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation, and survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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