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STAT4 Antibody (OTI2F3)

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Western Blot: STAT4 Antibody (2F3) [NBP2-00851] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT4.
Western Blot: STAT4 Antibody (2F3) [NBP2-00851] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: STAT4 Antibody (2F3) [NBP2-00851] Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT4.
Immunohistochemistry-Paraffin: STAT4 Antibody (2F3) [NBP2-00851] - Staining of paraffin-embedded Human tonsil using anti-STAT4 mouse monoclonal antibody.
Flow Cytometry: STAT4 Antibody (2F3) [NBP2-00851] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-STAT4 antibody, and then analyzed ...read more
Immunohistochemistry-Paraffin: STAT4 Antibody (2F3) [NBP2-00851] - Staining of paraffin-embedded Human lymphoma tissue using anti-STAT4 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74389AF350
Alexa Fluor 405
NBP2-74389AF405
Alexa Fluor 488
NBP2-74389AF488
Alexa Fluor 532
NBP2-74389AF532
Alexa Fluor 594
NBP2-74389AF594
Alexa Fluor 647
NBP2-74389AF647
Alexa Fluor 700
NBP2-74389AF700
Alexa Fluor 750
NBP2-74389AF750
Allophycocyanin
NBP2-74389APC
Allophycocyanin/Cy7
NBP2-74389APCCY7
Biotin
NBP2-74389B
CoraFluor 1
NBP2-74389CL1
DyLight 350
NBP2-74389UV
DyLight 405
NBP2-74389V
DyLight 488
NBP2-74389G
DyLight 550
NBP2-74389R
DyLight 594
NBP2-74389DL594
DyLight 650
NBP2-74389C
DyLight 680
NBP2-74389FR
DyLight 755
NBP2-74389IR
FITC
NBP2-74389F
HRP
NBP2-74389H
Janelia Fluor 525
NBP2-74389JF525
Janelia Fluor 549
NBP2-74389JF549
Janelia Fluor 646
NBP2-74389JF646
Janelia Fluor 669
NBP2-74389JF669
PE
NBP2-74389PE
PE/Atto594
NBP2-74389PEATT594
PE/Cy5.5
NBP2-74389PECY55
PE/Cy7
NBP2-74389PECY7
PerCP
NBP2-74389PCP
mFluor Violet 450 SE
NBP2-74389MFV450
mFluor Violet 500 SE
NBP2-74389MFV500
mFluor Violet 610 SE
NBP2-74389MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74389
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STAT4 Antibody (OTI2F3) Summary

Immunogen
Full length human recombinant protein of human STAT4(NP_003142) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STAT4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
85.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STAT4 Antibody (OTI2F3)

  • signal transducer and activator of transcription 4
  • SLEB11
  • STAT4

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STAT4
Entrez