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STAT1 Antibody (OTI15H3)

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Western Blot: STAT1 Antibody (15H3) [NBP1-48013] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1.
Western Blot: STAT1 Antibody (15H3) [NBP1-48013] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: STAT1 Antibody (15H3) [NBP1-48013] Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1.
Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-STAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-STAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-STAT1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI15H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.6 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74377AF350
Alexa Fluor 405
NBP2-74377AF405
Alexa Fluor 488
NBP2-74377AF488
Alexa Fluor 532
NBP2-74377AF532
Alexa Fluor 594
NBP2-74377AF594
Alexa Fluor 647
NBP2-74377AF647
Alexa Fluor 700
NBP2-74377AF700
Alexa Fluor 750
NBP2-74377AF750
Biotin
NBP2-74377B
CoraFluor 1
NBP2-74377CL1
DyLight 350
NBP2-74377UV
DyLight 405
NBP2-74377V
DyLight 488
NBP2-74377G
DyLight 550
NBP2-74377R
DyLight 594
NBP2-74377DL594
DyLight 650
NBP2-74377C
DyLight 680
NBP2-74377FR
DyLight 755
NBP2-74377IR
FITC
NBP2-74377F
HRP
NBP2-74377H
Janelia Fluor 525
NBP2-74377JF525
Janelia Fluor 549
NBP2-74377JF549
Janelia Fluor 646
NBP2-74377JF646
Janelia Fluor 669
NBP2-74377JF669
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74377
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STAT1 Antibody (OTI15H3) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human STAT1 expression vector
Specificity
This antibody is specific for Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant beta.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.6 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STAT1 Antibody (OTI15H3)

  • CANDF7
  • DKFZp686B04100
  • ISGF-3
  • ISGF-3,91kD
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription-1
  • STAT1
  • STAT91

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STAT1
Entrez