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SPAG9 Antibody

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Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Analysis in human testis and skeletal muscle tissues using NBP1-85393 antibody. Corresponding SPAG9 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Analysis in human testis and skeletal muscle tissues using NBP1-85393 antibody. Corresponding SPAG9 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: SPAG9 Antibody [NBP1-85393] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & microtubule organizing center.
Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Staining of human Liver shows very weak cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Staining of human Lymph node shows very weak cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Staining of human Testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SPAG9 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSN
Predicted Species
Mouse (92%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPAG9
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SPAG9 Protein (NBP1-85393PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SPAG9 Antibody

  • Cancer/testis antigen 89
  • c-Jun NH2-terminal kinase-associated leucine zipper protein
  • C-Jun-amino-terminal kinase-interacting protein 4
  • CT89JIP4
  • FLJ14006
  • FLJ26141
  • FLJ34602
  • HLC4
  • HLC-6
  • HSS
  • Human lung cancer oncogene 6 protein
  • JLPPHETSYD1FLJ13450
  • JNK interacting protein
  • JNK/SAPK-associated protein
  • JNK-associated leucine-zipper protein
  • JNK-interacting protein 4
  • KIAA0516JIP-4
  • lung cancer oncogene 4
  • MAPK8IP4
  • Max-binding protein
  • MGC117291
  • MGC14967
  • MGC74461
  • Mitogen-activated protein kinase 8-interacting protein 4
  • PIG6
  • proliferation-inducing gene 6
  • Proliferation-inducing protein 6
  • Protein highly expressed in testis
  • sperm associated antigen 9
  • Sperm surface protein
  • Sperm-associated antigen 9
  • Sperm-specific protein
  • Sunday driver 1

Background

Extracellular signals are transduced into cells through mitogen-activated protein kinases. The structural organization of these kinases into specific signaling domains is facilitated by scaffolding proteins involved in closely tethering different kinases so that successive phosphorylation events can occur. The protein encoded by this gene is a scaffolding protein that brings together mitogen-activated protein kinases and their transcription factor targets for the activation of specific signaling pathways. This gene which is abundantly expressed in testicular haploid germ cells encodes a protein that is recognized by sperm-agglutinating antibodies and implicated in infertility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SPAG9 Antibody
NBP1-85393
SPAG9 Antibody
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPAG9