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CDO Antibody [Unconjugated]

 (12 Publications)
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Western blot shows lysate of C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CDO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2429) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CDO at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysate of C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CDO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2429) followed by HRP-conjugated ...read more
PKN2 levels are elevated during myoblast differentiation and decreased in Cdo-depleted cells with a concurrent reduction in AKT activation. (a) C2C12 cells were cultured to near confluency (D0) and induced to ...read more
PKN2 levels are elevated during myoblast differentiation and decreased in Cdo-depleted cells with a concurrent reduction in AKT activation. (a) C2C12 cells were cultured to near confluency (D0) and induced to ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2429
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2429-SP
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CDO Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CDO
Val22-Leu963
Accession # AAC43031
Specificity
Detects mouse CDO in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDON
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF2429 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDO Antibody [Unconjugated]

  • CDO
  • Cdon homolog (mouse)
  • CDON
  • CDOORCAM
  • cell adhesion molecule-related/down-regulated by oncogenes
  • MGC111524
  • ORCAM
  • surface glycoprotein, Ig superfamily member

Background

Mouse CDO is a 190 kDa member of the Ig/FN-type III repeat subfamily of transmembrane (TM) proteins. The extracellular region contains five Ig-like domains and three FN-III modules. The molecule forms cis-complexes with itself and the related molecule termed BOC. Mouse CDO extracellular domain shares 95% and 85% aa identity with the corresponding regions of rat and human CDO, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDO Antibody (AF2429)(12)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IHC
(2)
IHC-P
(1)
Western Blot
(3)
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Mouse
(6)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF2429 Applications Species
X Li, PJ Gordon, JA Gaynes, AW Fuller, R Ringuette, CP Santiago, V Wallace, S Blackshaw, P Li, EM Levine Lhx2 is a progenitor-intrinsic modulator of Sonic Hedgehog signaling during early retinal neurogenesis Elife, 2022-12-02;11(0):. 2022-12-02 [PMID: 36459481] (IHC, Mouse) IHC Mouse
F Okumura, N Oki, Y Fujiki, R Ikuta, K Osaki, S Hamada, K Nakatsukas, N Hisamoto, T Hara, T Kamura ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation Scientific Reports, 2021-10-22;11(1):20880. 2021-10-22 [PMID: 34686700] (ICC, Western Blot, Mouse) ICC, Western Blot Mouse
S Lim, YJ Kim, S Park, JH Choi, Y Sung, K Nishimori, Z Kozmik, HW Lee, JW Kim mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent M�ller glia Elife, 2021-10-22;10(0):. 2021-10-22 [PMID: 34677125] (IHC, Mouse) IHC Mouse
Candice Chapouly, Pierre-Louis Hollier, Sarah Guimbal, Lauriane Cornuault, Alain-Pierre Gadeau, Marie-Ange Renault Desert Hedgehog-Driven Endothelium Integrity Is Enhanced by Gas1 (Growth Arrest-Specific 1) but Negatively Regulated by Cdon (Cell Adhesion Molecule-Related/Downregulated by Oncogenes) Arteriosclerosis, Thrombosis, and Vascular Biology 12/1/2020 [PMID: 33028094]
Moon K H, Kim H T et al. Differential Expression of NF2 in Neuroepithelial Compartments Is Necessary for Mammalian Eye Development. Dev Cell 8/1/2018 [PMID: 29249622]
Sang-Jin Lee, Jeongmi Hwang, Hyeon-Ju Jeong, Miran Yoo, Ga-Yeon Go, Jae-Rin Lee et al. PKN2 and Cdo interact to activate AKT and promote myoblast differentiation Cell Death & Disease 10/20/2016 [PMID: 27763641]
Dehydrocorydaline promotes myogenic differentiation via p38�MAPK activation Mol Med Rep, 2016-08-19;14(4):3029-36. 2016-08-19 [PMID: 27573543] (Western Blot, Mouse) Western Blot Mouse
Michael Verwey, Alanna Grant, Nicholas Meti, Lauren Adye-White, Angelica Torres-Berrío, Veronique Rioux et al. Mesocortical Dopamine Phenotypes in Mice Lacking the Sonic Hedgehog Receptor Cdon eNeuro 6/29/2016 [PMID: 27419218]
Yoo M, Kim B, Lee S, Jeong H, Park J, Seo D, Kim Y, Lee H, Han J, Kang J, Bae G Syntaxin 4 regulates the surface localization of a promyogenic receptor Cdo thereby promoting myogenic differentiation. Skelet Muscle, 2015-09-07;5(0):28. 2015-09-07 [PMID: 26347807] (Western Blot, Mouse) Western Blot Mouse
Jane Y. Song, Alexander M. Holtz, Justine M. Pinskey, Benjamin L. Allen Distinct structural requirements for CDON and BOC in the promotion of Hedgehog signaling Developmental Biology 6/15/2015 [PMID: 25848697]
Show All 12 Publications.

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Bioinformatics

Gene Symbol CDON
Uniprot