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SOCS-3 Antibody (OTI3D3)

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Western Blot: SOCS3 Antibody (3D3) [NBP2-00817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.
Western Blot: SOCS3 Antibody (3D3) [NBP2-00817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: SOCS3 Antibody (3D3) [NBP2-00817] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3.
Immunohistochemistry-Paraffin: SOCS3 Antibody (3D3) [NBP2-00817] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOCS3 mouse monoclonal antibody.
Flow Cytometry: SOCS3 Antibody (3D3) [NBP2-00817] - Analysis of Jurkat cells, using anti-SOCS3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: SOCS3 Antibody (3D3) [NBP2-00817] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SOCS3 mouse monoclonal antibody.
Flow Cytometry: SOCS3 Antibody (3D3) [NBP2-00817] - Analysis of Hela cells, using anti-SOCS3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74276AF350
Alexa Fluor 405
NBP2-74276AF405
Alexa Fluor 488
NBP2-74276AF488
Alexa Fluor 532
NBP2-74276AF532
Alexa Fluor 594
NBP2-74276AF594
Alexa Fluor 647
NBP2-74276AF647
Alexa Fluor 700
NBP2-74276AF700
Alexa Fluor 750
NBP2-74276AF750
Allophycocyanin
NBP2-74276APC
Allophycocyanin/Cy7
NBP2-74276APCCY7
Biotin
NBP2-74276B
CoraFluor 1
NBP2-74276CL1
DyLight 350
NBP2-74276UV
DyLight 405
NBP2-74276V
DyLight 488
NBP2-74276G
DyLight 550
NBP2-74276R
DyLight 594
NBP2-74276DL594
DyLight 650
NBP2-74276C
DyLight 680
NBP2-74276FR
DyLight 755
NBP2-74276IR
FITC
NBP2-74276F
HRP
NBP2-74276H
Janelia Fluor 525
NBP2-74276JF525
Janelia Fluor 549
NBP2-74276JF549
Janelia Fluor 585
NBP2-74276JF585
Janelia Fluor 635
NBP2-74276JF635
Janelia Fluor 646
NBP2-74276JF646
Janelia Fluor 669
NBP2-74276JF669
PE
NBP2-74276PE
PE/Atto594
NBP2-74276PEATT594
PE/Cy5.5
NBP2-74276PECY55
PE/Cy7
NBP2-74276PECY7
PerCP
NBP2-74276PCP
mFluor Violet 450 SE
NBP2-74276MFV450
mFluor Violet 500 SE
NBP2-74276MFV500
mFluor Violet 610 SE
NBP2-74276MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74276
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SOCS-3 Antibody (OTI3D3) Summary

Immunogen
Full length human recombinant protein of human SOCS3 (NP_003946) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SOCS3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
24.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOCS-3 Antibody (OTI3D3)

  • ATOD4
  • CIS3
  • CIS3CIS-3
  • Cish3
  • cytokine-induced SH2 protein 3
  • Cytokine-inducible SH2 protein 3
  • SOCS3
  • SOCS-3
  • SOCS-3STAT-induced STAT inhibitor 3
  • SSI-3
  • SSI-3ATOD4
  • SSI3MGC71791
  • suppressor of cytokine signaling 3

Background

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SOCS3
Entrez