Home » Smad2 » Smad2 ELISA Kits » Rat Smad2 ELISA Kit (Colorimetric)

Rat Smad2 ELISA Kit (Colorimetric)

 Submit a Review

Images

 
(
Enlarge)
ELISA: Rat Smad2 ELISA Kit (Colorimetric) [NBP2-76619] - Samples were spiked with high concentrations of Rat Smad2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Smad2 ELISA Kit (Colorimetric) [NBP2-76619] - Samples were spiked with high concentrations of Rat Smad2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat Smad2 ELISA Kit (Colorimetric) [NBP2-76619] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.156 - 10ng/mL
Sensitivity
0.094ng/mL

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Rat Smad2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Smad2/MADH2 in samples. No significant cross-reactivity or interference between Rat Smad2/MADH2 and analogues was observed.
Standard Curve Range
0.156 - 10ng/mL
Sensitivity
0.094ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.72
Intra-Assay
CV% < 5.88
Spike Recovery
84-104%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SMAD2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Smad2 ELISA Kit (Colorimetric)

  • hMAD-2
  • hSMAD2
  • JV18
  • JV18-1
  • JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
  • MAD homolog 2
  • Mad protein homolog
  • Mad, mothers against decapentaplegic homolog 2
  • MADH2
  • MADH2MGC34440
  • MADR2
  • MADR2MGC22139
  • Mad-related protein 2
  • mother against DPP homolog 2
  • mothers against decapentaplegic homolog 2
  • Mothers against DPP homolog 2
  • Sma- and Mad-related protein 2
  • SMAD 2
  • SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
  • SMAD, mothers against DPP homolog 2
  • Smad2
  • Smox

Background

Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Smad3 Antibody
NB100-56479
Immunocytochemistry/ Immunofluorescence Smad3 Antibody
Western Blot Smad3 Antibody
Immunohistochemistry-Paraffin Smad3 Antibody
(5 Reviews)
(8 Publications)
Species: Bv, Ca, Hu, Mu, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Smad4 Antibody
[Unconjugated]
AF2097
Chromatin Immunoprecipitation (ChIP) Smad4 Antibody [Unconjugated]
Western Blot Smad4 Antibody [Unconjugated]
Immunocytochemistry Smad4 Antibody [Unconjugated]
(9 Publications)
Species: Hu
Applications: ChIP, ICC, WB
Smad7 Antibody
(293039) [Unconjugate
MAB2029
Western Blot Smad7 Antibody (293039) [Unconjugated]
Immunohistochemistry Smad7 Antibody (293039) [Unconjugated]
Immunohistochemistry Smad7 Antibody (293039) [Unconjugated]
(1 Review)
(59 Publications)
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
TGF-beta 1
[Unconjugated]
7754-BH/CF
Bioactivity TGF-beta 1 [Unconjugated]
(53 Publications)
Species: Hu
Applications: BA
TGF-beta RI/ALK-5
Antibody [Unconjugate ...
AF3025
Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]
Immunocytochemistry TGF-beta RI/ALK-5 Antibody [Unconjugated]
Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]
(16 Publications)
Species: Hu
Applications: ELISA, ICC, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Smad1 Antibody
[Unconjugated]
AF2039
Immunohistochemistry Smad1 Antibody [Unconjugated]
Western Blot Smad1 Antibody [Unconjugated]
Simple Western Smad1 Antibody [Unconjugated]
(8 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
GARS Antibody
NBP3-35329
Western Blot GARS Antibody
Species: Hu, Mu
Applications: ELISA, ICC/IF, WB
CTGF/CCN2 Antibody
[Unconjugated] - C-te ...
AF660
Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus
Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus
Western Blot CTGF/CCN2 Antibody [Unconjugated] - C-terminus
(7 Publications)
Species: Hu
Applications: IHC
Fibronectin
Antibody - BSA Free
NBP1-91258
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
(11 Reviews)
(56 Publications)
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin p21/CIP1/CDKN1A Antibody (WA-1)
(24 Publications)
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Serpin E1/PAI-1
Antibody - BSA Free
NBP1-19773
Western Blot Serpin E1/PAI-1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Serpin E1/PAI-1 Antibody - BSA Free
Immunohistochemistry-Paraffin Serpin E1/PAI-1 Antibody - BSA Free
(2 Reviews)
(32 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Nodal
[Unconjugated]
3218-ND
Nodal [Unconjugated]
(22 Publications)
Species: Hu
Applications: BA
EphB2 Antibody
[Unconjugated]
AF467
Simple Western EphB2 Antibody [Unconjugated]
Western Blot EphB2 Antibody [Unconjugated]
Immunocytochemistry EphB2 Antibody [Unconjugated]
(5 Reviews)
(63 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
JNK1 Antibody
(228601) [Unconjugate
MAB17761
Western Blot JNK1 Antibody (228601) [Unconjugated]
Western Blot JNK1 Antibody (228601) [Unconjugated]
Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC, WB
Rat Smad2 ELISA
Kit (Colorimetric)
NBP2-76619
Rat Smad2 ELISA Kit (Colorimetric)
Species: Rt
Applications: ELISA

Publications for Smad2 ELISA Kit (NBP2-76619) (0)

There are no publications for Smad2 ELISA Kit (NBP2-76619).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Smad2 ELISA Kit (NBP2-76619) (0)

There are no reviews for Smad2 ELISA Kit (NBP2-76619). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Smad2 ELISA Kit (NBP2-76619) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Smad2 Products

Research Areas for Smad2 ELISA Kit (NBP2-76619)

Find related products by research area.

Blogs on Smad2.

Check out the latest blog posts on Smad2.
TGF-beta for treating degenerative intervertebral disc disease
By Jamshed Arslan Pharm.D. Our upright posture and balance depend on a jelly-like material, called nucleus pulposus (NP), in the middle of intervertebral discs. NP cells protect us from disc degeneration by maintain...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  SDS
  Rat Smad2 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Rat Smad2 ELISA Kit (Colorimetric) and receive a gift card or discount.


Bioinformatics

Gene Symbol SMAD2