Smad2 Antibodies
We offer SMAD2 Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each SMAD2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SMAD2 Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Human, Mouse, Primate, Rat. Use the list below to choose the SMAD2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SMAD2 polyclonal antibodies and browse our SMAD2 monoclonal antibody catalog.
Alternate Names for Smad2 Antibodies
anti-Smad2 antibody, anti-hMAD-2 antibody, anti-hSMAD2 antibody, anti-JV18 antibody, anti-JV18-1SMAD, mothers against DPP homolog 2 (Drosophila) antibody, anti-MAD homolog 2 antibody, anti-Mad protein homolog antibody, anti-Mad, mothers against decapentaplegic homolog 2 antibody, anti-Mad-related protein 2 antibody, anti-MADH2MGC34440 antibody, anti-MADR2MGC22139 antibody, anti-mother against DPP homolog 2 antibody, anti-mothers against decapentaplegic homolog 2 antibody, anti-Mothers against DPP homolog 2 antibody, anti-Sma- and Mad-related protein 2 antibody, anti-SMAD 2 antibody, anti-SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila) antibody, anti-SMAD, mothers against DPP homolog 2 antibody
17 Results for "smad2" in Primary Antibodies
![Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Western-Blot-NB100-56462-img0004.jpg "Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: Smad2 Antibody [NB100-56462] - Mouse testis tissue probed with this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Immunohistochemistry-Paraffin-NB100-56462-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human fallopian tube and pancreas tissues using NBP2-88916 antibody. Corresponding SMAD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human fallopian tube and pancreas tissues using NBP2-88916 antibody. Corresponding SMAD2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human cell line U-138MG.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Western-Blot-NBP2-88916-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of HeLa cells using the Anti-SMAD2 monoclonal antibody, showing specific staining in the cytosol and nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunocytochemistry-Immunofluorescence-NBP2-88916-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human breast cancer shows moderate cytoplasmic positivity.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human Fallopian tube shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human pancreas shows low cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0004.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human testis shows weak to moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0006.jpg)
![Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against HEK293 (1) and SMAD2(AA: 20-254)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Western-Blot-NBP2-37580-img0003.jpg "Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against HEK293 (1) and SMAD2(AA: 20-254)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (5G7) [NBP2-37580] - Immunofluorescence analysis of U251 cells using SMAD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Immunocytochemistry-Immunofluorescence-NBP2-37580-img0004.jpg)
![Immunohistochemistry: Smad2 Antibody (5G7) [NBP2-37580] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using SMAD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Immunohistochemistry-NBP2-37580-img0007.jpg)
![Flow Cytometry: Smad2 Antibody (5G7) [NBP2-37580] - Flow cytometric analysis of NIH/3T3 cells using SMAD2 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Flow-Cytometry-NBP2-37580-img0005.jpg)
![Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against human SMAD2 (AA: 20-254) recombinant protein. (Expected MW is 52.2 kDa)](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Western-Blot-NBP2-37580-img0002.jpg)
![Immunohistochemistry: Smad2 Antibody (5G7) [NBP2-37580] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using SMAD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Immunohistochemistry-NBP2-37580-img0006.jpg)
![ELISA: Smad2 Antibody (5G7) [NBP2-37580] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-ELISA-NBP2-37580-img0001.jpg)
![Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extract from Hela cells untreated or treated with IL-4(100ng/mL, 30min), using Smad2 (Ab-220) Antibody (NBP1-04981, Lanes 1 and 2) and Smad2 (Phospho-Thr220) Antibody (NBP1-04982, Lanes 3 and 4).](http://images.novusbio.com/fullsize/Smad2-[p-Thr220]-Antibody-Western-Blot-NBP1-04982-img0004.jpg "Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extract from Hela cells untreated or treated with IL-4(100ng/mL, 30min), using Smad2 (Ab-220) Antibody (NBP1-04981, Lanes 1 and 2) and Smad2 (Phospho-Thr220) Antibody (NBP1-04982, Lanes 3 and 4).")
![Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extracts from Hela cells untreated or treeated with PMAA using Smadd2 (Phospho-Thhr220) Antibody .](http://images.novusbio.com/fullsize/Smad2-[p-Thr220]-Antibody-Western-Blot-NBP1-04982-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad2 [p Thr220] Antibody [NBP1-04982] - Immunohistochemical analysis of Smad2 staining in human brain formalin fixed paraffin embedded tissue section.](http://images.novusbio.com/fullsize/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-234202415232511.jpg)
![Immunocytochemistry/ Immunofluorescence: Smad2 [p Thr220] Antibody [NBP1-04982] - Immunofluorescent analysis of Smad2 staining in HeLa cells.](http://images.novusbio.com/fullsize/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-234202414462213.jpg)
![Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Western blot analysis of Smad2 [p Thr220] expression in rat heart (A) whole cell lysates.](http://images.novusbio.com/fullsize/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-23420241452107.jpg)
![Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of Rat lung, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Western-Blot-NBP3-15671-img0004.jpg "Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of Rat lung, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (2E3D8) [NBP3-15671] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad2 Rabbit mAb (NBP3-15671) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Immunocytochemistry-Immunofluorescence-NBP3-15671-img0002.jpg)
![Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of various cell lines, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Western-Blot-NBP3-15671-img0003.jpg)
![Immunoprecipitation: Smad2 Antibody (2E3D8) [NBP3-15671] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Smad2 antibody (NBP3-15671). Western blot was performed from the immunoprecipitate using Smad2 antibody (NBP3-15671) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Immunoprecipitation-NBP3-15671-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Smad2 Antibody (2E3D8) [Smad2] - Western blot analysis of lysates from wild type (WT) and Smad2 knockdown (KD) HeLa cells using [KD Validated] Smad2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15671_rabbit-smad2-mab-2e3d8-52202517304995.jpg)
![Immunocytochemistry/ Immunofluorescence: Smad2 Antibody (2E3D8) [Smad2] - Confocal imaging of HeLa cells using [KD Validated] Smad2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15671_rabbit-smad2-mab-2e3d8-522025173455.jpg)
![Western Blot: Smad2 [p Ser467] Antibody [NBP3-16891] - Western blot analysis of extracts of HeLa cells, using Smad2 antibody (NBP3-16891) at 1:1000 dilution.Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Smad2-[p-Ser467]-Antibody-Western-Blot-NBP3-16891-img0001.jpg "Western Blot: Smad2 [p Ser467] Antibody [NBP3-16891] - Western blot analysis of extracts of HeLa cells, using Smad2 antibody (NBP3-16891) at 1:1000 dilution.Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Rat testis tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125711..jpg)
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Mouse testis tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125830..jpg)
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Human kidney tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125949..jpg)
![Western Blot: Smad2 Antibody [NBP3-02957] - Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (NBP3-02957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.](http://images.novusbio.com/fullsize/Smad2-Antibody-Western-Blot-NBP3-02957-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Smad2 Antibody [NBP3-02957] - Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (NBP3-02957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.")
![Western Blot: Smad2 [p Ser467] Antibody [NBP2-54771] - Analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad2 (Phospho-Ser467).](http://images.novusbio.com/fullsize/Smad2-[p-Ser467]-Antibody-Western-Blot-NBP2-54771-img0001.jpg "Western Blot: Smad2 [p Ser467] Antibody [NBP2-54771] - Analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad2 (Phospho-Ser467).")
![Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273] - Untreated (-) and treated (+) MCF-7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser465/Ser467) antibody (NBP3-13273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad2-[p-Ser465--p-Ser467]-Antibody-Western-Blot-NBP3-13273-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273] - Untreated (-) and treated (+) MCF-7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser465/Ser467) antibody (NBP3-13273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273] - Smad2 (phospho Ser465/Ser467) antibody detects Smad2 (phospho Ser465/Ser467) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Smad2 (phospho Ser465/Ser467) stained by Smad2 (phospho Ser465/Ser467) antibody (NBP3-13273) diluted at 1:1500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Smad2-[p-Ser465--p-Ser467]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13273-img0002.jpg)