Slit2 Antibody (710305) [Alexa Fluor® 647]

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Slit2 is a 180-210 kDa secreted member of the SLIT family of proteins. It is expressed in select sites in the embryo (via glia, motoneurons and posterior sclerotome mesoderm), and found in discrete adult cell types such as preosteoblasts, monocytes, granulose lutein cells, and likely keratinocytes. Slit2 binds to multiple receptors, including ROBO-1 thru -4, Laminin-1, Dan, Gremlin and Netrin-1. Depending upon the target, Slit2 can promote a number of diverse effects, including both growth cone collapse and outgrowth, inhibition of dendritic cell migration, and axon repulsion. Mature mouse Slit2 is 1496 amino acids (aa) in length (aa 26-1521). It contains multiple intermingled domains, including nine EGF-like domains, 20 Leu-rich repeats (LRRs), one laminin G-like and CTCK (C-terminal Cys knot-like) domain, and eight total C- plus N-terminal LRRs. There are two potential isoform splice variants. One contains a four aa insertion after Ser258, while another possesses the same insertion after Ser258 coupled to both an eight aa insertion after Ser479 and a nine aa insertion after Thr1021. Slit2 apparently undergoes proteolytic cleavage after Arg1113. This generates a 140-150 kDa N-terminal protein, and a 55-60 kDa C-terminal fragment. This processing does not inactivate Slit2. Rather, it creates molecules with distinct activities. For example, the N-terminal fragment will bind ROBO1 and repel motor axon migration, while the C-terminal fragment won't bind ROBO1, but will bind glypican-1 and promote motor axon migration. Over aa 26-900, mouse Slit2 shares 99% and 97% aa sequence identity with rat and human Slit2, respectively.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Array FAB5444R

• Slit2 Antibodies
• Slit2 ELISA Kits
• Slit2 Lysate
• Slit2 Proteins
• Slit2 Proteins and Enzymes