Slit2 Antibodies
We offer Slit2 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each Slit2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Slit2 Antibodies can be used in a variety of model species: Bovine, Chicken, Human, Mouse, Rat, Xenopus. Use the list below to choose the Slit2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Slit2 polyclonal antibodies and browse our Slit2 monoclonal antibody catalog.
Alternate Names for Slit2 Antibodies
anti-Slit2 antibody, anti-FLJ14420 antibody, anti-slit (Drosophila) homolog 2 antibody, anti-slit homolog 2 (Drosophila) antibody, anti-slit homolog 2 protein antibody, anti-Slit-2SLIL3 antibody
17 Results for "slit2" in Primary Antibodies
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Paraffin-embedded mouse fetal brain. Green: SLIT2 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP2-20398-img0018.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Paraffin-embedded mouse fetal brain. Green: SLIT2 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.")
![Western Blot: Slit2 Antibody [NBP2-20398] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/Slit2-Antibody-Western-Blot-NBP2-20398-img0016.jpg)
![Western Blot: Slit2 Antibody [NBP2-20398] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Slit2-Antibody-Western-Blot-NBP2-20398-img0021.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP2-20398-img0011.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Rat brain. SLIT2 antibody diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP2-20398-img0015.jpg)
![Immunoprecipitation: Slit2 Antibody [NBP2-20398] - Raji whole cell lysate/extract A : 30 ug whole cell lysate/extract of SLIT2 protein expressing Raji cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of SLIT2 by 3 ug of SLIT2 antibody 5% SDS-PAGE The immunoprecipitated SLIT2 protein was detected by SLIT2 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunoprecipitation-NBP2-20398-img0012.jpg)
![Western Blot: Slit2 Antibody [NBP2-20398] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Slit2 antibody (NBP2-20398) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20398_rabbit-polyclonal-slit2-antibody-910202420115092.jpg)
![Western Blot: Slit2 Antibody [NBP2-20398] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Slit2 antibody (NBP2-20398) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20398_rabbit-polyclonal-slit2-antibody-910202420122024.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80742] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP1-80742-img0004.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80742] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.")
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80742] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP1-80742-img0001.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80742] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP1-80742-img0002.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80742] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP1-80742-img0003.jpg)
![Western Blot: Slit2 Antibody (JE31-87) [NBP3-32955] - Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: PC-3M cell lysate (15 ug/Lane) Lane 4: Raji cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32955) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-288202412461662.jpg "Western Blot: Slit2 Antibody (JE31-87) [NBP3-32955] - Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: PC-3M cell lysate (15 ug/Lane) Lane 4: Raji cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32955) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Slit2 Antibody (JE31-87) [NBP3-32955] - Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202410484851.jpg)
![Flow Cytometry: Slit2 Antibody (JE31-87) [NBP3-32955] - Flow cytometric analysis of A549 cells labeling Slit2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32955, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202416372174.jpg)
![Western Blot: Slit2 Antibody (1T5A3) [NBP3-16194] - Western blot analysis of extracts of various cell lines, using Slit2 Rabbit mAb (NBP3-16194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Slit2-Antibody-1T5A3-Western-Blot-NBP3-16194-img0002.jpg "Western Blot: Slit2 Antibody (1T5A3) [NBP3-16194] - Western blot analysis of extracts of various cell lines, using Slit2 Rabbit mAb (NBP3-16194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Slit2 Antibody (1T5A3) [NBP3-16194] - Immunohistochemistry of paraffin-embedded rat ovary using Slit2 Rabbit mAb (NBP3-16194) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Slit2-Antibody-1T5A3-Immunohistochemistry-Paraffin-NBP3-16194-img0001.jpg)
![Western Blot: Slit2 Antibody [NB600-881] - Analysis of a band at 165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).](http://images.novusbio.com/fullsize/Slit2-Antibody-Western-Blot-NB600-881-img0009.jpg "Western Blot: Slit2 Antibody [NB600-881] - Analysis of a band at 165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).")
![Immunohistochemistry: Slit2 Antibody [NB600-881] - Analysis of human breast carcinoma Tissue: Human prostate Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker, EDTA pH 9.5 Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45” RT](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-NB600-881-img0005.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807] - Staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NB100-40807-img0001.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807] - Staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80743] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP1-80743-img0003.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80743] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.")
![Western Blot: Slit2 Antibody (SR2034) [NBP3-21803] - Western blot analysis using NBP3-21803 in SH-SY5Y cell lysate.](http://images.novusbio.com/fullsize/nbp3-21803_rabbit-slit2-mab-sr2034-57202316494277.jpg "Western Blot: Slit2 Antibody (SR2034) [NBP3-21803] - Western blot analysis using NBP3-21803 in SH-SY5Y cell lysate.")